Detailed information NTG0766365

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O4443_RS04665
• Genome accession: NZ_CP114551
• Coordinates: 1046212..1047342 (-)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa subsp. pauca strain OLI17A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1041212..1052342

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O4443_RS04645 (O4443_04645)	-	1041283..1042041 (+)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-
O4443_RS04650 (O4443_04650)	hemC	1042058..1042975 (+)	918	WP_010894123.1	hydroxymethylbilane synthase	-
O4443_RS04655 (O4443_04655)	-	1043029..1043838 (-)	810	WP_031336664.1	DUF481 domain-containing protein	-
O4443_RS04660 (O4443_04660)	-	1044682..1045695 (+)	1014	WP_229716473.1	YafY family protein	-
O4443_RS04665 (O4443_04665)	pilU	1046212..1047342 (-)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O4443_RS04670 (O4443_04670)	pilT	1047508..1048542 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
O4443_RS04675 (O4443_04675)	-	1049805..1050632 (+)	828	WP_173405994.1	glycosyltransferase family 2 protein	-
O4443_RS04680 (O4443_04680)	-	1051296..1052120 (-)	825	WP_088372032.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=766365 O4443_RS04665 WP_010894128.1 1046212..1047342(-) (pilU) [Xylella fastidiosa subsp. pauca strain OLI17A]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=766365 O4443_RS04665 WP_010894128.1 1046212..1047342(-) (pilU) [Xylella fastidiosa subsp. pauca strain OLI17A]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACTCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399