Detailed information NTG0766349

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O4445_RS04680
• Genome accession: NZ_CP114550
• Coordinates: 1045888..1047018 (-)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa subsp. pauca strain AM2-Angelina
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1040888..1052018

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O4445_RS04660 (O4445_04660)	-	1040959..1041717 (+)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-
O4445_RS04665 (O4445_04665)	hemC	1041734..1042651 (+)	918	WP_010894123.1	hydroxymethylbilane synthase	-
O4445_RS04670 (O4445_04670)	-	1042705..1043514 (-)	810	WP_031336664.1	DUF481 domain-containing protein	-
O4445_RS04675 (O4445_04675)	-	1044358..1045371 (+)	1014	WP_229716473.1	YafY family protein	-
O4445_RS04680 (O4445_04680)	pilU	1045888..1047018 (-)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O4445_RS04685 (O4445_04685)	pilT	1047184..1048218 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
O4445_RS04690 (O4445_04690)	-	1048287..1048484 (-)	198	WP_010894129.1	hypothetical protein	-
O4445_RS04695 (O4445_04695)	-	1049481..1050308 (+)	828	WP_173405994.1	glycosyltransferase family 2 protein	-
O4445_RS04700 (O4445_04700)	-	1050972..1051796 (-)	825	WP_088372032.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=766349 O4445_RS04680 WP_010894128.1 1045888..1047018(-) (pilU) [Xylella fastidiosa subsp. pauca strain AM2-Angelina]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=766349 O4445_RS04680 WP_010894128.1 1045888..1047018(-) (pilU) [Xylella fastidiosa subsp. pauca strain AM2-Angelina]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACTCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399