Detailed information NTG0076528

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H7K53_RS04675
• Genome accession: NZ_AP022077
• Coordinates: 970626..971744 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain WP4-W18-ESBL-11
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 965626..976744

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7K53_RS04645 (WP4W18E11_08840)	-	966593..967561 (+)	969	WP_005804963.1	IS30 family transposase	-
H7K53_RS04650	-	967562..968011 (+)	450	WP_182639124.1	bacteriohemerythrin	-
H7K53_RS04655 (WP4W18E11_08860)	-	968164..969231 (+)	1068	WP_004736014.1	hypothetical protein	-
H7K53_RS04660 (WP4W18E11_08870)	-	969231..969554 (+)	324	WP_000442582.1	RnfH family protein	-
H7K53_RS04665 (WP4W18E11_08880)	-	969609..970007 (-)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
H7K53_RS04670 (WP4W18E11_08890)	fur	970119..970556 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H7K53_RS04675 (WP4W18E11_08900)	pilU	970626..971744 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H7K53_RS04680 (WP4W18E11_08910)	pilT	971772..972809 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H7K53_RS04685 (WP4W18E11_08920)	-	972936..973628 (+)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=76528 H7K53_RS04675 WP_000347039.1 970626..971744(-) (pilU) [Acinetobacter baumannii strain WP4-W18-ESBL-11]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=76528 H7K53_RS04675 WP_000347039.1 970626..971744(-) (pilU) [Acinetobacter baumannii strain WP4-W18-ESBL-11]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395