Detailed information NTG0076376

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: LDO79_RS02135
• Genome accession: NZ_AP024984
• Coordinates: 387274..388248 (-)
• Length: 324 a.a.
• NCBI ID: WP_011227913.1
• Uniprot ID: Q5SL87
• Organism: Thermus thermophilus strain JCM10941
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 382274..393248

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDO79_RS02115 (JCM10941_04050)	-	382530..382958 (+)	429	WP_011227910.1	hypothetical protein	-
LDO79_RS02120 (JCM10941_04060)	ftsH	382944..384800 (-)	1857	WP_011227911.1	ATP-dependent zinc metalloprotease FtsH	-
LDO79_RS02125 (JCM10941_04070)	-	384873..386225 (+)	1353	WP_008631640.1	tetratricopeptide repeat protein	-
LDO79_RS02130 (JCM10941_04080)	-	386246..387277 (-)	1032	WP_011172495.1	DUF4388 domain-containing protein	-
LDO79_RS02135 (JCM10941_04090)	ruvB	387274..388248 (-)	975	WP_011227913.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
LDO79_RS02140 (JCM10941_04100)	panB	388274..389059 (+)	786	WP_011227914.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
LDO79_RS02145 (JCM10941_04110)	-	389107..390033 (+)	927	WP_011227915.1	RluA family pseudouridine synthase	-
LDO79_RS02150 (JCM10941_04120)	-	390030..390923 (+)	894	WP_011227916.1	site-specific DNA-methyltransferase	-
LDO79_RS02155 (JCM10941_04130)	-	390898..391587 (+)	690	WP_164926029.1	hypothetical protein	-
LDO79_RS02160 (JCM10941_04140)	lysN	391561..392754 (-)	1194	WP_011227918.1	2-aminoadipate transaminase	-

Sequence

Protein

• Length: 324 a.a.
• Molecular weight: 35973.74 Da
• Isoelectric Point: 6.3232

>NTDB_id=76376 LDO79_RS02135 WP_011227913.1 387274..388248(-) (ruvB) [Thermus thermophilus strain JCM10941]
MEDLALRPKTLDEYIGQERLKQKLRVYLEAAKARKEPLEHLLLFGPPGLGKTTLAHVIAHELGVNLRVTSGPAIEKPGDL
AAILANSLEEGDILFIDEIHRLSRQAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAPLLSRFG
IVEHLEYYTPEELAQGVMRDARLLGVRITEEAALEIGRRSRGTMRVAKRLFRRVRDFAQVAGEEVITRERALEALAALGL
DELGLEKRDREILEVLILRFGGGPVGLATLATALSEDPGTLEEVHEPYLIRQGLLKRTPRGRVATELAYRHLGYPPPVGP
LLEP

Nucleotide

• Length: 975 bp

>NTDB_id=76376 LDO79_RS02135 WP_011227913.1 387274..388248(-) (ruvB) [Thermus thermophilus strain JCM10941]
GTGGAAGACCTCGCCCTTAGGCCCAAGACCCTGGACGAGTACATCGGCCAGGAGCGCCTGAAGCAAAAGCTCCGGGTCTA
CCTCGAGGCGGCCAAGGCCCGAAAAGAGCCCTTGGAGCACCTCCTCCTCTTCGGCCCCCCGGGCCTGGGCAAGACCACCC
TGGCCCACGTCATCGCCCACGAGCTTGGGGTCAACCTCCGGGTCACCTCCGGGCCCGCCATAGAGAAGCCCGGGGACCTC
GCCGCCATCCTGGCCAACTCCCTGGAGGAAGGGGACATCCTCTTCATTGACGAGATCCACCGCCTGAGCCGCCAGGCCGA
GGAGCACCTCTACCCCGCCATGGAGGACTTCGTCATGGACATCGTCATCGGCCAAGGTCCGGCGGCGAGGACCATCCGGC
TGGAGCTTCCCCGCTTCACCCTGATCGGGGCCACCACCCGGCCCGGCCTCATCACCGCGCCCCTCCTGAGCCGCTTCGGC
ATCGTGGAGCACCTGGAGTACTACACCCCTGAGGAGCTGGCCCAAGGGGTGATGCGGGACGCCCGCCTCCTCGGGGTGAG
GATCACGGAGGAGGCCGCCCTGGAGATCGGTAGGCGGAGCCGGGGCACCATGCGCGTCGCCAAGCGCCTCTTCCGGAGGG
TGCGGGACTTCGCCCAGGTGGCGGGGGAGGAGGTCATCACCCGGGAGCGGGCCCTGGAGGCCCTTGCCGCCCTGGGCCTG
GACGAGCTGGGCTTGGAGAAGCGGGACCGGGAGATCCTGGAGGTCCTCATCCTCCGCTTCGGCGGCGGCCCCGTGGGCCT
CGCCACCCTGGCCACCGCCCTCTCCGAGGACCCGGGCACCTTGGAAGAGGTGCACGAGCCCTACCTCATCCGCCAGGGCC
TTTTGAAGCGCACTCCCCGGGGCCGGGTGGCCACGGAACTGGCCTACCGCCACCTGGGCTACCCGCCCCCGGTGGGCCCC
CTCTTGGAGCCGTGA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (168-241)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (6-165)

• RuvB C-terminal winged helix domain (WHD_RuvB) (242-312)

3D structure

• PDB: 1HQC
• PDB: 1IXR
• PDB: 1IXS
• PDB: 8EFV
• PDB: 8EFY

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.105	96.605	0.571
ruvB	Streptococcus pneumoniae TIGR4	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae R6	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae D39	56.774	95.679	0.543
ruvB	Synechocystis sp. PCC 6803	50.779	99.074	0.503
ruvB	Helicobacter pylori 26695	50.637	96.914	0.491