Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OU800_RS13820 Genome accession   NZ_CP113519
Coordinates   3148335..3149537 (-) Length   400 a.a.
NCBI ID   WP_268177868.1    Uniprot ID   -
Organism   Pseudomonas sp. GOM7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3143335..3154537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU800_RS13805 (OU800_13805) - 3144380..3145279 (+) 900 WP_268177865.1 hydroxymethylglutaryl-CoA lyase -
  OU800_RS13810 (OU800_13810) - 3145470..3147425 (+) 1956 WP_268177866.1 acetoacetate--CoA ligase -
  OU800_RS13815 (OU800_13815) - 3147589..3148338 (+) 750 WP_268177867.1 transporter substrate-binding domain-containing protein -
  OU800_RS13820 (OU800_13820) pilU 3148335..3149537 (-) 1203 WP_268177868.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OU800_RS13825 (OU800_13825) - 3149685..3149963 (+) 279 WP_017362973.1 peptidylprolyl isomerase -
  OU800_RS13830 (OU800_13830) - 3150205..3152100 (+) 1896 WP_268177869.1 patatin-like phospholipase family protein -
  OU800_RS13835 (OU800_13835) - 3153256..3154194 (+) 939 WP_268177870.1 L-threonylcarbamoyladenylate synthase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 44151.39 Da        Isoelectric Point: 6.4422

>NTDB_id=762685 OU800_RS13820 WP_268177868.1 3148335..3149537(-) (pilU) [Pseudomonas sp. GOM7]
MDLHAMLKVLSTQDGSDLYLSTGAPPCAKFNGVLKALSQEPLKSGDVAAIAESIMDGAQREEFERELEMNLAISLPGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLSTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTADGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLHRDNPANAKPAPVAAAPAAPAKAAAASEWELKLEEIEQEQPPEDPGRHGI

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=762685 OU800_RS13820 WP_268177868.1 3148335..3149537(-) (pilU) [Pseudomonas sp. GOM7]
ATGGATCTCCATGCAATGCTCAAGGTGCTGTCCACTCAGGATGGTTCCGACCTGTACCTCTCCACGGGCGCACCGCCTTG
CGCCAAGTTCAATGGCGTGCTCAAGGCGCTCAGCCAGGAGCCCTTGAAGTCCGGCGACGTGGCGGCCATCGCCGAGTCGA
TCATGGACGGTGCGCAACGCGAGGAATTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGCTGCCGGGCGTCGGGCGT
TTCCGTATCAATATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGCGCTTCGA
GGATCTCAAGCTGCCCGAAGTGCTGCTCAGCACGGTGATGGAGAAGCGTGGCCTGGTACTGTTCGTCGGTGGCACCGGCT
CGGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAAGTTGGGGTGGACACTCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGTGACCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATTTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAATTTCTTCCCCGAAGAACGTCGTCCGCAACTGCTCAACGATCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTCAAGACCGCCGATGGCAAGCGTCGCGCCGCCGTGGAGGTGATGCTGGGCACGCCGACGGTGCGCGATCTGATCA
AGCGCAACGAGTTCTCCGAGCTCAAGGAAATCATGGAGAAGTCGAAGAACCTGGGCATGCAGACCTTCGATCAGGCGTTG
ATCGACCTGGTGCACGAAGGTGCCATCGACGAGGAAGAGGCGGTGAAGAATGCCGACTCGGCGAACAACGTGCGCCTCAA
GCTCAAGCTGCACCGCGACAATCCAGCCAATGCCAAGCCGGCGCCTGTCGCCGCTGCACCCGCGGCGCCTGCGAAAGCGG
CAGCAGCCAGTGAGTGGGAGCTGAAGCTGGAGGAGATTGAGCAGGAGCAGCCCCCGGAAGATCCGGGGCGCCACGGCATC
TAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.84

87.75

0.49
  pilU Vibrio cholerae strain A1552

52.408

88.25

0.463
  pilU Acinetobacter baylyi ADP1

51.705

88

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.323

93

0.375