Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NM686_RS01640 Genome accession   NZ_CP113517
Coordinates   359518..360579 (+) Length   353 a.a.
NCBI ID   WP_255190209.1    Uniprot ID   -
Organism   Methylomonas rapida strain MP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354518..365579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM686_RS01625 (NM686_001625) - 355196..356557 (+) 1362 WP_255190206.1 AmpG family muropeptide MFS transporter -
  NM686_RS01630 (NM686_001630) rpmE 356663..356863 (+) 201 WP_255190207.1 50S ribosomal protein L31 -
  NM686_RS01635 (NM686_001635) - 356932..359307 (-) 2376 WP_269022232.1 penicillin-binding protein 1A -
  NM686_RS01640 (NM686_001640) pilM 359518..360579 (+) 1062 WP_255190209.1 pilus assembly protein PilM Machinery gene
  NM686_RS01645 (NM686_001645) - 360579..361151 (+) 573 WP_255190210.1 PilN domain-containing protein -
  NM686_RS01650 (NM686_001650) - 361148..361771 (+) 624 WP_255190211.1 type 4a pilus biogenesis protein PilO -
  NM686_RS01655 (NM686_001655) - 361930..362433 (+) 504 WP_269022895.1 pilus assembly protein PilP -
  NM686_RS01660 (NM686_001660) pilQ 362511..364790 (+) 2280 WP_255190213.1 type IV pilus secretin family protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38404.12 Da        Isoelectric Point: 4.8065

>NTDB_id=762643 NM686_RS01640 WP_255190209.1 359518..360579(+) (pilM) [Methylomonas rapida strain MP1]
MSWFNRNQSALLGIDISTAAVKLLELSRTGARYKVESYAVAPLPQDAIIDKNITNVEVIGNAIKAAVKQSGTRAKRACVA
VAGSSVMTKIISMPATLSETDMEEQIMIEADQYIPYSLDEVNLDFEVQGVSRNNPEMLDVLLAASRRENIEDRVAALAYA
GLKTAIVDVEAFAMENAFTLLMDQLPEKMENKTVAIADIGATMTTLNILHDGKTIYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYIPDVLEPFKKAMLQQIARSLQFFVSSSANRGVDALILAGGCSSIPGLDKLVERDLGIPSYIANPF
INMALSSRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=762643 NM686_RS01640 WP_255190209.1 359518..360579(+) (pilM) [Methylomonas rapida strain MP1]
ATGAGCTGGTTTAACAGGAATCAGTCTGCCTTGCTCGGTATCGATATCAGCACGGCAGCGGTCAAATTATTGGAGTTAAG
CCGAACTGGCGCGCGCTACAAGGTAGAAAGCTATGCCGTCGCGCCGCTTCCGCAGGATGCGATCATCGATAAAAACATCA
CCAACGTAGAAGTCATAGGTAACGCCATCAAGGCTGCCGTAAAACAATCGGGGACACGCGCCAAGCGGGCTTGCGTGGCC
GTGGCCGGGTCGTCGGTCATGACAAAAATCATTTCCATGCCTGCTACGTTGAGCGAGACGGACATGGAAGAACAGATCAT
GATCGAGGCCGATCAATATATTCCCTATTCTCTGGATGAAGTAAATCTCGACTTCGAGGTGCAAGGCGTTTCACGAAACA
ATCCGGAGATGTTGGACGTCTTGTTGGCCGCCTCGCGGCGAGAGAATATCGAGGATCGCGTGGCTGCATTGGCCTATGCG
GGCTTGAAAACAGCCATCGTGGATGTCGAGGCCTTTGCCATGGAAAATGCCTTTACGCTGCTGATGGATCAATTGCCTGA
AAAAATGGAAAACAAAACCGTCGCAATCGCCGATATTGGGGCCACGATGACGACGTTGAACATTTTGCACGACGGCAAAA
CCATCTATACCCGCGAGCAGGGTTTCGGCGGCAAACAGCTCACCGAAGAGATCCAGCGTCGTTACGGTCTTTCGTATGAA
GAAGCCGGTTTGGCCAAAAAGCATGGCGGATTGCCGGACAACTATATTCCCGATGTCTTGGAACCGTTTAAAAAAGCGAT
GTTGCAACAAATCGCCCGATCCTTGCAGTTTTTTGTTTCCTCCAGCGCAAACCGGGGTGTGGATGCGTTGATTTTGGCCG
GTGGCTGTTCCTCGATACCGGGGTTGGACAAGCTGGTCGAGCGTGATTTGGGTATTCCGTCTTATATTGCCAATCCTTTC
ATCAACATGGCGTTGTCGAGTCGAGTCAAGCCACAAAGTTTGAGTAACGATACGCCCGCGATGATGATCGCATGCGGCTT
GGCATTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.858

100

0.499
  comM Acinetobacter nosocomialis M2

49.858

100

0.499
  comM Acinetobacter baylyi ADP1

47.875

100

0.479
  pilM Legionella pneumophila strain ERS1305867

42.21

100

0.422