Detailed information NTG0762463

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OWC53_RS15420
• Genome accession: NZ_CP113504
• Coordinates: 3344537..3345382 (-)
• Length: 281 a.a.
• NCBI ID: WP_268197550.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain 21PCA_AGRO2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3339537..3350382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OWC53_RS15395 (OWC53_15380)	-	3339756..3340526 (-)	771	WP_268197546.1	ABC transporter ATP-binding protein	-
OWC53_RS15400 (OWC53_15385)	-	3340528..3341547 (-)	1020	WP_268197547.1	iron ABC transporter permease	-
OWC53_RS15405 (OWC53_15390)	-	3341544..3342608 (-)	1065	WP_268197548.1	ABC transporter substrate-binding protein	-
OWC53_RS15410 (OWC53_15395)	-	3342918..3343943 (+)	1026	WP_268197549.1	LLM class flavin-dependent oxidoreductase	-
OWC53_RS15415 (OWC53_15400)	ypdK	3344301..3344375 (+)	75	WP_099606229.1	membrane protein YpdK	-
OWC53_RS15420 (OWC53_15405)	pilD	3344537..3345382 (-)	846	WP_268197550.1	A24 family peptidase	Machinery gene
OWC53_RS15425 (OWC53_15410)	-	3345472..3346218 (-)	747	WP_268197551.1	type II secretion system protein N	-
OWC53_RS15430 (OWC53_15415)	-	3346208..3346702 (-)	495	WP_268197552.1	type II secretion system protein M	-
OWC53_RS15435 (OWC53_15420)	gspL	3346699..3347976 (-)	1278	WP_268197553.1	type II secretion system protein GspL	-
OWC53_RS15440 (OWC53_15425)	gspK	3347973..3348959 (-)	987	WP_268197554.1	type II secretion system minor pseudopilin GspK	-
OWC53_RS15445 (OWC53_15430)	gspJ	3348956..3349606 (-)	651	WP_268197555.1	type II secretion system minor pseudopilin GspJ	-
OWC53_RS15450 (OWC53_15435)	gspI	3349606..3349971 (-)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31413.37 Da
• Isoelectric Point: 5.7656

>NTDB_id=762463 OWC53_RS15420 WP_268197550.1 3344537..3345382(-) (pilD) [Pectobacterium brasiliense strain 21PCA_AGRO2]
MDDLREFAQLFPAWWFGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=762463 OWC53_RS15420 WP_268197550.1 3344537..3345382(-) (pilD) [Pectobacterium brasiliense strain 21PCA_AGRO2]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCGGCGTGGTGGTTTGGGGCGCTGGGCGTGTTGGGGTTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATGGCCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAGCAGGCTATT
GCAGTAAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGTTGCTGCCATCAATCGGTGTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTACCCGGTATGGCGCTGT
GGGGCGCGTTGATTCTGCTGTCCTTCCTGTTGGTGCTAACCGTGATTGATATCAAAACGCTGCTGCTGCCGGATGTACTT
ACGCTGTCGCTTCTGTGGATGGGGCTGCTGTTTAATCTGTCGGAGACATTTGTGCCATTGAATGATGCCGTCGTCGGCGC
GATGGCCGGTTATTTATCTTTGTGGCTACTTTATTGGGTATTCAAATACGCCACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCACTGGGTGCCTGGCTGGGTTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGTTGAGC
GGGTTGGTCGTTACTCTGGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCTTTTGGCCCCTGGCTGGC
AATGGGCGGGGTGTTCGGCGTGATAATGAATGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.185	96.441	0.484
pilD	Vibrio campbellii strain DS40M4	45.556	96.085	0.438
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Acinetobacter baumannii D1279779	42.125	97.153	0.409
pilD	Acinetobacter nosocomialis M2	41.758	97.153	0.406