Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5R32_RS22075 Genome accession   NZ_CP113493
Coordinates   4563309..4564289 (-) Length   326 a.a.
NCBI ID   WP_001361345.1    Uniprot ID   A0A2U2VV72
Organism   Escherichia coli strain GTEN_97     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4558309..4569289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5R32_RS22045 yggI 4558935..4559432 (+) 498 WP_023908971.1 SprT family zinc-dependent metalloprotease -
  E5R32_RS22050 endA 4559527..4560234 (+) 708 WP_000286500.1 deoxyribonuclease I -
  E5R32_RS22055 rsmE 4560314..4561045 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E5R32_RS22060 gshB 4561058..4562008 (+) 951 WP_000593278.1 glutathione synthase -
  E5R32_RS22065 yqgE 4562117..4562680 (+) 564 WP_001339287.1 YqgE/AlgH family protein -
  E5R32_RS22070 ruvX 4562680..4563096 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  E5R32_RS22075 pilT 4563309..4564289 (-) 981 WP_001361345.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5R32_RS22080 yggS 4564307..4565011 (+) 705 WP_000997793.1 pyridoxal phosphate homeostasis protein -
  E5R32_RS22085 yggT 4565029..4565595 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  E5R32_RS22090 yggU 4565592..4565882 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  E5R32_RS22095 rdgB 4565890..4566483 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  E5R32_RS22100 hemW 4566476..4567612 (+) 1137 WP_000239986.1 radical SAM family heme chaperone HemW -
  E5R32_RS22105 - 4567925..4568911 (+) 987 WP_001564022.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35970.18 Da        Isoelectric Point: 5.7980

>NTDB_id=762410 E5R32_RS22075 WP_001361345.1 4563309..4564289(-) (pilT) [Escherichia coli strain GTEN_97]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAVPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=762410 E5R32_RS22075 WP_001361345.1 4563309..4564289(-) (pilT) [Escherichia coli strain GTEN_97]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGTGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTTACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2VV72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368