Detailed information NTG0761953

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OU419_RS13440
• Genome accession: NZ_CP113432
• Coordinates: 2876852..2878084 (+)
• Length: 410 a.a.
• NCBI ID: WP_254474425.1
• Uniprot ID: -
• Organism: Pseudomonas triclosanedens strain ZM23
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2871852..2883084

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OU419_RS13415 (OU419_13415)	-	2872506..2874152 (+)	1647	WP_254474434.1	phosphoethanolamine transferase	-
OU419_RS13420 (OU419_13420)	-	2874131..2875330 (-)	1200	WP_254474432.1	MFS transporter	-
OU419_RS13425 (OU419_13425)	-	2875470..2876024 (-)	555	WP_254474431.1	putative glycolipid-binding domain-containing protein	-
OU419_RS13430 (OU419_13430)	-	2876021..2876386 (-)	366	WP_254474429.1	MmcQ/YjbR family DNA-binding protein	-
OU419_RS13435 (OU419_13435)	-	2876389..2876667 (-)	279	WP_254474427.1	peptidylprolyl isomerase	-
OU419_RS13440 (OU419_13440)	pilU	2876852..2878084 (+)	1233	WP_254474425.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OU419_RS13445 (OU419_13445)	-	2878259..2879143 (+)	885	WP_254474423.1	DMT family transporter	-
OU419_RS13450 (OU419_13450)	-	2879150..2879968 (-)	819	WP_254474421.1	helix-turn-helix domain-containing protein	-
OU419_RS13455 (OU419_13455)	-	2880136..2881578 (+)	1443	WP_254474419.1	sodium:alanine symporter family protein	-
OU419_RS13460 (OU419_13460)	-	2881663..2882655 (+)	993	WP_254474416.1	asparaginase	-

Sequence

Protein

• Length: 410 a.a.
• Molecular weight: 44712.13 Da
• Isoelectric Point: 6.1281

>NTDB_id=761953 OU419_RS13440 WP_254474425.1 2876852..2878084(+) (pilU) [Pseudomonas triclosanedens strain ZM23]
MDIQSMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSGEPMKAGDVARIGYGLMDEEQRAEFDRELEMNLAISIQGVGR
FRINLFKQRNEVSIVARNIKLDIPQFDDLKLPEVLLKVIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDLIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLQRDNPAMAMSAAAAKAAPAPAPAPMASATPAVAAAEASNWGLELALEEIE
AEDADKPGGL

Nucleotide

• Length: 1233 bp

>NTDB_id=761953 OU419_RS13440 WP_254474425.1 2876852..2878084(+) (pilU) [Pseudomonas triclosanedens strain ZM23]
ATGGATATCCAGTCGATGCTGAAGATCCTGGCCACCCAGGATGGTTCCGATCTCTACCTCTCCACCGGCGCGCCGCCTTG
CGCCAAGTTCAACGGGGTGCTCAAGCCGCTGTCCGGCGAGCCGATGAAGGCTGGCGATGTGGCACGCATCGGCTATGGCC
TGATGGACGAGGAGCAACGCGCCGAGTTCGACCGCGAGCTGGAGATGAACCTGGCCATTTCCATTCAGGGCGTAGGCCGT
TTCCGGATCAACCTGTTCAAGCAGCGCAACGAAGTCTCCATCGTGGCGCGCAACATCAAGCTCGACATCCCGCAGTTCGA
CGACCTCAAGCTGCCGGAAGTGCTGCTCAAGGTGATCATGGAGAAGCGTGGGCTGGTGCTGTTCGTCGGTGGCACCGGCT
CTGGCAAGTCCACCTCGCTGGCGGCCCTGATCGACCACCGCAACCGCAATAGCGGCGGGCACATCATCACCATCGAAGAC
CCGATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAAGTGGGCGTGGATACGCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCAGATGTGATCCTGATCGGCGAGATTCGCGACCGCGAGACGATGGAACACG
CCCTGGCCTTCGCCGATACCGGCCACCTGGCCATTTCCACCCTGCATGCAAACAACGCCAACCAGGCTCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTGCGTACCCAGGACGGCAAGCGCCGCGCGGCGGTGGAAGTGCTGCTGGGCACCGCCACCATCAGCGATCTGATCA
AGCGCGGTGACTTCAGCTCGATCAAGGAAATCATGGACAAGTCCCGTGCGCTGGGCATGCAGACCTTCGACCAGGCACTG
TTCGACCTTGCGGTGGAGGGTGCCATCAGCGAGGAAGAGGCCGTGAAAAACGCCGACTCGGCGAACAATCTGCGCCTGAA
GCTCAAGCTGCAGCGCGACAACCCGGCGATGGCCATGAGCGCTGCCGCGGCCAAGGCGGCACCAGCGCCGGCACCGGCTC
CGATGGCATCTGCCACGCCGGCCGTCGCTGCCGCCGAAGCTTCCAATTGGGGGCTGGAACTGGCGCTGGAGGAGATCGAA
GCCGAGGATGCGGACAAACCCGGCGGCCTGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	85.61	0.488
pilU	Acinetobacter baylyi ADP1	53.693	85.854	0.461
pilU	Vibrio cholerae strain A1552	51.558	86.098	0.444
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.265	95.61	0.366