Detailed information NTG0761324

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSV15_RS22145
• Genome accession: NZ_CP113257
• Coordinates: 4609289..4610434 (-)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain TF18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4604289..4615434

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSV15_RS22120 (OSV15_22120)	-	4604372..4605388 (+)	1017	WP_267931427.1	aspartate carbamoyltransferase catalytic subunit	-
OSV15_RS22125 (OSV15_22125)	-	4605388..4606662 (+)	1275	WP_267931428.1	dihydroorotase	-
OSV15_RS22130 (OSV15_22130)	-	4606717..4607112 (-)	396	WP_017245432.1	NINE protein	-
OSV15_RS22135 (OSV15_22135)	-	4607244..4608458 (+)	1215	WP_267931429.1	putative DNA modification/repair radical SAM protein	-
OSV15_RS22140 (OSV15_22140)	-	4608458..4609282 (+)	825	WP_267931430.1	TIGR03915 family putative DNA repair protein	-
OSV15_RS22145 (OSV15_22145)	pilU	4609289..4610434 (-)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OSV15_RS22150 (OSV15_22150)	pilT	4610495..4611529 (-)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
OSV15_RS22155 (OSV15_22155)	-	4611596..4612285 (+)	690	WP_267931431.1	YggS family pyridoxal phosphate-dependent enzyme	-
OSV15_RS22160 (OSV15_22160)	proC	4612296..4613120 (+)	825	WP_267931432.1	pyrroline-5-carboxylate reductase	-
OSV15_RS22165 (OSV15_22165)	-	4613130..4613720 (+)	591	WP_017245439.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=761324 OSV15_RS22145 WP_026006585.1 4609289..4610434(-) (pilU) [Stutzerimonas frequens strain TF18]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=761324 OSV15_RS22145 WP_026006585.1 4609289..4610434(-) (pilU) [Stutzerimonas frequens strain TF18]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCACCGGAGATGACCCGCGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATTGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACGATGGACTATG
CCGTAGCCTTCGCCGAGACCGGGCACCTGTGTCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCACCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACACCCGACGGCAAGGGTCGTCGCGCGGTGATCGAGGTGTTGATCAACACCCCGCTGGCCGCGGACCTTA
TCCGCAAGGGCGAGGTGCATGAACTCAAGTCGCTGATGAAACGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTATGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTGATCAAGCTGGGTTCGGAAACCGACGGCGAGCACCTGACCACTGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388