Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ORP33_RS12755 Genome accession   NZ_CP113256
Coordinates   2486317..2486934 (-) Length   205 a.a.
NCBI ID   WP_122894878.1    Uniprot ID   -
Organism   Bacillus subtilis strain K1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2481317..2491934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORP33_RS12725 (ORP33_12485) rpsT 2481619..2481885 (+) 267 WP_131227637.1 30S ribosomal protein S20 -
  ORP33_RS12730 (ORP33_12490) holA 2481900..2482943 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  ORP33_RS12735 - 2482983..2483132 (-) 150 WP_003229985.1 hypothetical protein -
  ORP33_RS12740 (ORP33_12495) yqzM 2483173..2483307 (+) 135 WP_003229983.1 YqzM family protein -
  ORP33_RS12745 (ORP33_12500) comEC 2483347..2485677 (-) 2331 WP_213414606.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ORP33_RS12750 (ORP33_12505) comEB 2485681..2486250 (-) 570 WP_003229978.1 ComE operon protein 2 -
  ORP33_RS12755 (ORP33_12510) comEA 2486317..2486934 (-) 618 WP_122894878.1 competence protein ComEA Machinery gene
  ORP33_RS12760 (ORP33_12515) comER 2487018..2487839 (+) 822 WP_213414605.1 late competence protein ComER -
  ORP33_RS12765 (ORP33_12520) yqeM 2487905..2488648 (-) 744 WP_015384134.1 class I SAM-dependent DNA methyltransferase -
  ORP33_RS12770 (ORP33_12525) rsfS 2488645..2489001 (-) 357 WP_014477391.1 ribosome silencing factor -
  ORP33_RS12775 (ORP33_12530) yqeK 2489019..2489579 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ORP33_RS12780 (ORP33_12535) nadD 2489569..2490138 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ORP33_RS12785 (ORP33_12540) yhbY 2490150..2490440 (-) 291 WP_213414604.1 ribosome assembly RNA-binding protein YhbY -
  ORP33_RS12790 (ORP33_12545) aroE 2490434..2491276 (-) 843 WP_076458133.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21827.58 Da        Isoelectric Point: 4.9552

>NTDB_id=761271 ORP33_RS12755 WP_122894878.1 2486317..2486934(-) (comEA) [Bacillus subtilis strain K1]
MNWLNQHKKTIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSKQADEAQVNLAEVLQDGTVVYIPKKGEETAVQQGTGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=761271 ORP33_RS12755 WP_122894878.1 2486317..2486934(-) (comEA) [Bacillus subtilis strain K1]
ATGAATTGGTTGAATCAGCATAAGAAAACAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCTATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAGTAAACAAGCAGACGAAGCGCAAGTAAATTTGGCCGA
GGTTCTTCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTACCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGGCCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.073

100

0.971
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

36.364

100

0.39