Detailed information NTG0760956

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSZ60_RS06105
• Genome accession: NZ_CP113230
• Coordinates: 1253847..1254995 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain BIAI 160
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1248847..1259995

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSZ60_RS06085 (OSZ60_06085)	-	1249585..1250769 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
OSZ60_RS06090 (OSZ60_06090)	-	1250766..1252139 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
OSZ60_RS06095 (OSZ60_06095)	-	1252382..1252786 (-)	405	WP_003084560.1	NINE protein	-
OSZ60_RS06100 (OSZ60_06100)	czcD	1252940..1253839 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
OSZ60_RS06105 (OSZ60_06105)	pilU	1253847..1254995 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
OSZ60_RS06110 (OSZ60_06110)	pilT	1255173..1256207 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
OSZ60_RS06115 (OSZ60_06115)	-	1256422..1257114 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
OSZ60_RS06120 (OSZ60_06120)	proC	1257126..1257947 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
OSZ60_RS06125 (OSZ60_06125)	-	1257958..1258551 (+)	594	WP_003084544.1	YggT family protein	-
OSZ60_RS06130 (OSZ60_06130)	-	1258548..1258844 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=760956 OSZ60_RS06105 WP_003100959.1 1253847..1254995(-) (pilU) [Pseudomonas aeruginosa strain BIAI 160]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=760956 OSZ60_RS06105 WP_003100959.1 1253847..1254995(-) (pilU) [Pseudomonas aeruginosa strain BIAI 160]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGATCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382