Detailed information NTG0760261

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSZ59_RS24875
• Genome accession: NZ_CP113106
• Coordinates: 5308296..5309444 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain BIAI 157
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5303296..5314444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSZ59_RS24855 (OSZ59_24855)	-	5304034..5305218 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
OSZ59_RS24860 (OSZ59_24860)	-	5305215..5306588 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
OSZ59_RS24865 (OSZ59_24865)	-	5306831..5307235 (-)	405	WP_003084560.1	NINE protein	-
OSZ59_RS24870 (OSZ59_24870)	czcD	5307389..5308288 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
OSZ59_RS24875 (OSZ59_24875)	pilU	5308296..5309444 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
OSZ59_RS24880 (OSZ59_24880)	pilT	5309622..5310656 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
OSZ59_RS24885 (OSZ59_24885)	-	5310871..5311563 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
OSZ59_RS24890 (OSZ59_24890)	proC	5311575..5312396 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
OSZ59_RS24895 (OSZ59_24895)	-	5312407..5313000 (+)	594	WP_003084544.1	YggT family protein	-
OSZ59_RS24900 (OSZ59_24900)	-	5312997..5313293 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=760261 OSZ59_RS24875 WP_003100959.1 5308296..5309444(-) (pilU) [Pseudomonas aeruginosa strain BIAI 157]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=760261 OSZ59_RS24875 WP_003100959.1 5308296..5309444(-) (pilU) [Pseudomonas aeruginosa strain BIAI 157]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGATCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382