Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OSS47_RS20500 Genome accession   NZ_CP113096
Coordinates   4558429..4559631 (+) Length   400 a.a.
NCBI ID   WP_176707187.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain G5Li.T10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 4560122..4561225 4558429..4559631 flank 491


Gene organization within MGE regions


Location: 4558429..4561225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSS47_RS20500 (OSS47_20500) pilU 4558429..4559631 (+) 1203 WP_176707187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OSS47_RS20505 (OSS47_20505) - 4560122..4561207 (-) 1086 WP_267853868.1 IS110 family transposase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 43903.04 Da        Isoelectric Point: 6.0779

>NTDB_id=760161 OSS47_RS20500 WP_176707187.1 4558429..4559631(+) (pilU) [Pseudomonas citronellolis strain G5Li.T10]
MEIQAMLRILSSQDGSDLYLSTGAPPCAKFNGVLKPLSSEPLKAGDVARIADAIMDEEQKAEFERELEMNLAISVAGVGR
FRINIFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLHRDNPAAAQTQAASAQPATPAAPAVEASSWGLELALEEIKAEDEEDKKGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=760161 OSS47_RS20500 WP_176707187.1 4558429..4559631(+) (pilU) [Pseudomonas citronellolis strain G5Li.T10]
ATGGAAATCCAAGCGATGCTGCGGATCCTGTCCAGCCAGGACGGTTCCGACCTTTACCTTTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAGCGAACCGCTGAAGGCCGGCGACGTGGCGCGCATCGCCGACGCCA
TCATGGACGAGGAACAGAAGGCCGAATTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGGTCGCCGGCGTCGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGCTGTTCGA
AGACCTGAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACTTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCCATCGAATACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGCGCCGCGGTGGAGGTGCTGCTGGGCACCGCGACCATCAGCGACATCATCA
AGCGCGGCGACTTCAGCTCGATCAAGGAGATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGCGCGATCAGCGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGGCTGAA
GCTCAAGCTGCACCGGGACAACCCGGCTGCGGCCCAGACCCAGGCCGCCAGCGCCCAGCCAGCAACCCCGGCCGCGCCCG
CGGTGGAAGCCTCCAGCTGGGGCCTGGAACTGGCCCTGGAAGAGATCAAGGCCGAGGACGAGGAAGACAAGAAGGGCGGT
TGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.695

87.75

0.497
  pilU Acinetobacter baylyi ADP1

53.409

88

0.47
  pilU Vibrio cholerae strain A1552

52.125

88.25

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.157

95.25

0.382