Detailed information NTG0076007

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: LDO07_RS02245
• Genome accession: NZ_AP024937
• Coordinates: 404792..405766 (-)
• Length: 324 a.a.
• NCBI ID: WP_011227913.1
• Uniprot ID: Q5SL87
• Organism: Thermus thermophilus strain AK1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 399792..410766

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDO07_RS02225 (TthAK1_04280)	-	400048..400476 (+)	429	WP_011227910.1	hypothetical protein	-
LDO07_RS02230 (TthAK1_04290)	ftsH	400462..402318 (-)	1857	WP_143584724.1	ATP-dependent zinc metalloprotease FtsH	-
LDO07_RS02235 (TthAK1_04300)	-	402391..403743 (+)	1353	WP_014630116.1	tetratricopeptide repeat protein	-
LDO07_RS02240 (TthAK1_04310)	-	403764..404795 (-)	1032	WP_143584725.1	DUF4388 domain-containing protein	-
LDO07_RS02245 (TthAK1_04320)	ruvB	404792..405766 (-)	975	WP_011227913.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
LDO07_RS02250 (TthAK1_04330)	panB	405792..406577 (+)	786	WP_014630114.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
LDO07_RS02255 (TthAK1_04340)	-	406625..407551 (+)	927	WP_014630113.1	RluA family pseudouridine synthase	-
LDO07_RS02260 (TthAK1_04350)	-	407548..408441 (+)	894	WP_223965972.1	site-specific DNA-methyltransferase	-
LDO07_RS02265 (TthAK1_04360)	-	408416..409093 (+)	678	WP_172619159.1	hypothetical protein	-
LDO07_RS02270 (TthAK1_04370)	lysN	409079..410272 (-)	1194	WP_143584730.1	2-aminoadipate transaminase	-

Sequence

Protein

• Length: 324 a.a.
• Molecular weight: 35973.74 Da
• Isoelectric Point: 6.3232

>NTDB_id=76007 LDO07_RS02245 WP_011227913.1 404792..405766(-) (ruvB) [Thermus thermophilus strain AK1]
MEDLALRPKTLDEYIGQERLKQKLRVYLEAAKARKEPLEHLLLFGPPGLGKTTLAHVIAHELGVNLRVTSGPAIEKPGDL
AAILANSLEEGDILFIDEIHRLSRQAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAPLLSRFG
IVEHLEYYTPEELAQGVMRDARLLGVRITEEAALEIGRRSRGTMRVAKRLFRRVRDFAQVAGEEVITRERALEALAALGL
DELGLEKRDREILEVLILRFGGGPVGLATLATALSEDPGTLEEVHEPYLIRQGLLKRTPRGRVATELAYRHLGYPPPVGP
LLEP

Nucleotide

• Length: 975 bp

>NTDB_id=76007 LDO07_RS02245 WP_011227913.1 404792..405766(-) (ruvB) [Thermus thermophilus strain AK1]
GTGGAAGACCTCGCCCTTAGGCCCAAGACCCTGGACGAGTACATCGGCCAGGAACGCCTGAAGCAAAAGCTCCGGGTCTA
CCTCGAGGCGGCCAAGGCCCGAAAAGAGCCCTTGGAGCACCTCCTCCTCTTCGGCCCCCCGGGCCTGGGCAAGACCACCC
TGGCCCACGTCATCGCCCACGAGCTTGGGGTCAACCTCCGGGTCACCTCCGGGCCCGCCATAGAGAAGCCCGGGGACCTC
GCCGCCATCCTGGCCAACTCCCTGGAGGAAGGGGACATCCTCTTCATTGACGAGATCCACCGCTTGAGCCGCCAGGCCGA
GGAGCACCTCTACCCCGCCATGGAGGACTTCGTCATGGACATCGTCATCGGCCAAGGTCCGGCGGCGAGAACCATCCGGC
TGGAGCTTCCCCGCTTCACCCTGATCGGGGCCACCACCCGGCCCGGCCTCATCACCGCGCCCCTCTTGAGCCGCTTCGGC
ATCGTGGAGCACCTGGAGTACTACACCCCCGAGGAGCTGGCCCAAGGGGTGATGCGGGACGCCCGCCTCCTCGGGGTGAG
GATCACGGAGGAGGCCGCCCTGGAGATCGGTAGGCGGAGCCGGGGCACCATGCGCGTCGCCAAGCGCCTCTTCCGGAGGG
TGCGGGACTTCGCCCAGGTGGCGGGGGAGGAGGTCATCACCCGGGAGCGGGCCCTGGAGGCCCTTGCGGCCCTGGGCCTG
GACGAGCTGGGCTTGGAGAAGCGGGACCGGGAGATCCTGGAGGTCCTCATCCTCCGCTTCGGCGGTGGCCCAGTGGGCCT
CGCCACCTTAGCCACCGCCCTCTCCGAGGACCCGGGCACCTTGGAAGAGGTGCACGAGCCCTACCTCATCCGCCAGGGCC
TTTTGAAGCGCACTCCCCGGGGCCGGGTGGCCACGGAACTGGCCTACCGCCACCTGGGCTACCCGCCCCCGGTGGGCCCC
CTCTTGGAGCCGTGA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (168-241)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (6-165)

• RuvB C-terminal winged helix domain (WHD_RuvB) (242-312)

3D structure

• PDB: 1HQC
• PDB: 1IXR
• PDB: 1IXS
• PDB: 8EFV
• PDB: 8EFY

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.105	96.605	0.571
ruvB	Streptococcus pneumoniae TIGR4	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae R6	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae D39	56.774	95.679	0.543
ruvB	Synechocystis sp. PCC 6803	50.779	99.074	0.503
ruvB	Helicobacter pylori 26695	50.637	96.914	0.491