Detailed information NTG0760029

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSV63_RS14350
• Genome accession: NZ_CP113078
• Coordinates: 2932635..2933753 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB5075
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2927635..2938753

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSV63_RS14320 (OSV63_14320)	-	2928464..2929312 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
OSV63_RS14325 (OSV63_14325)	-	2929565..2930020 (+)	456	WP_000782976.1	bacteriohemerythrin	-
OSV63_RS14330 (OSV63_14330)	-	2930173..2931240 (+)	1068	WP_000092403.1	hypothetical protein	-
OSV63_RS14335 (OSV63_14335)	-	2931240..2931563 (+)	324	WP_000442585.1	RnfH family protein	-
OSV63_RS14340 (OSV63_14340)	-	2931618..2932016 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OSV63_RS14345 (OSV63_14345)	fur	2932128..2932565 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OSV63_RS14350 (OSV63_14350)	pilU	2932635..2933753 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OSV63_RS14355 (OSV63_14355)	pilT	2933781..2934818 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
OSV63_RS14360 (OSV63_14360)	-	2934945..2935637 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=760029 OSV63_RS14350 WP_000347036.1 2932635..2933753(-) (pilU) [Acinetobacter baumannii strain AB5075]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=760029 OSV63_RS14350 WP_000347036.1 2932635..2933753(-) (pilU) [Acinetobacter baumannii strain AB5075]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392