Detailed information NTG0759927

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSH15_RS14910
• Genome accession: NZ_CP113074
• Coordinates: 3090505..3091623 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3085505..3096623

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSH15_RS14900 (OSH15_14915)	-	3088621..3089313 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
OSH15_RS14905 (OSH15_14920)	pilT	3089440..3090477 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OSH15_RS14910 (OSH15_14925)	pilU	3090505..3091623 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OSH15_RS14915 (OSH15_14930)	fur	3091693..3092130 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OSH15_RS14920 (OSH15_14935)	bamE	3092242..3092640 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OSH15_RS14925 (OSH15_14940)	-	3092695..3093018 (-)	324	WP_000442590.1	RnfH family protein	-
OSH15_RS14930 (OSH15_14945)	-	3093018..3094085 (-)	1068	WP_004748097.1	hypothetical protein	-
OSH15_RS14935 (OSH15_14950)	-	3094238..3094693 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OSH15_RS14940 (OSH15_14955)	-	3094946..3095794 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=759927 OSH15_RS14910 WP_000347034.1 3090505..3091623(+) (pilU) [Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=759927 OSH15_RS14910 WP_000347034.1 3090505..3091623(+) (pilU) [Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39