Detailed information NTG0759826

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSH16_RS13435
• Genome accession: NZ_CP113072
• Coordinates: 2871593..2872711 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain A118 DeltaH0N27_10820-30::kan
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2866593..2877711

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSH16_RS13425 (OSH16_13425)	-	2869709..2870401 (-)	693	WP_168726751.1	YggS family pyridoxal phosphate-dependent enzyme	-
OSH16_RS13430 (OSH16_13430)	pilT	2870528..2871565 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OSH16_RS13435 (OSH16_13435)	pilU	2871593..2872711 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OSH16_RS13440 (OSH16_13440)	fur	2872781..2873218 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OSH16_RS13445 (OSH16_13445)	bamE	2873330..2873728 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OSH16_RS13450 (OSH16_13450)	-	2873783..2874106 (-)	324	WP_045901052.1	RnfH family protein	-
OSH16_RS13455 (OSH16_13455)	-	2874106..2875173 (-)	1068	WP_168726750.1	hypothetical protein	-
OSH16_RS13460 (OSH16_13460)	-	2875326..2875781 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OSH16_RS13465 (OSH16_13465)	-	2876034..2876882 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=759826 OSH16_RS13435 WP_000347034.1 2871593..2872711(+) (pilU) [Acinetobacter baumannii strain A118 DeltaH0N27_10820-30::kan]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=759826 OSH16_RS13435 WP_000347034.1 2871593..2872711(+) (pilU) [Acinetobacter baumannii strain A118 DeltaH0N27_10820-30::kan]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAATGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCACAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39