Detailed information NTG0759767

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSH14_RS04135
• Genome accession: NZ_CP113071
• Coordinates: 877366..878484 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain A118 DeltaH0N27_10830::kan
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 872366..883484

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSH14_RS04105 (OSH14_04105)	-	873195..874043 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
OSH14_RS04110 (OSH14_04110)	-	874296..874751 (+)	456	WP_000782976.1	bacteriohemerythrin	-
OSH14_RS04115 (OSH14_04115)	-	874904..875971 (+)	1068	WP_168726750.1	hypothetical protein	-
OSH14_RS04120 (OSH14_04120)	-	875971..876294 (+)	324	WP_045901052.1	RnfH family protein	-
OSH14_RS04125 (OSH14_04125)	bamE	876349..876747 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OSH14_RS04130 (OSH14_04130)	fur	876859..877296 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OSH14_RS04135 (OSH14_04135)	pilU	877366..878484 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OSH14_RS04140 (OSH14_04140)	pilT	878512..879549 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OSH14_RS04145 (OSH14_04145)	-	879676..880368 (+)	693	WP_168726751.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=759767 OSH14_RS04135 WP_000347034.1 877366..878484(-) (pilU) [Acinetobacter baumannii strain A118 DeltaH0N27_10830::kan]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=759767 OSH14_RS04135 WP_000347034.1 877366..878484(-) (pilU) [Acinetobacter baumannii strain A118 DeltaH0N27_10830::kan]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAATGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCACAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39