Detailed information NTG0075951

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: LDO80_RS02370
• Genome accession: NZ_AP024933
• Coordinates: 424871..425845 (-)
• Length: 324 a.a.
• NCBI ID: WP_011227913.1
• Uniprot ID: Q5SL87
• Organism: Thermus thermophilus strain AA3-7
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 419871..430845

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDO80_RS02350 (TthAA37_04620)	-	420127..420555 (+)	429	WP_011227910.1	hypothetical protein	-
LDO80_RS02355 (TthAA37_04630)	ftsH	420541..422397 (-)	1857	WP_143584724.1	ATP-dependent zinc metalloprotease FtsH	-
LDO80_RS02360 (TthAA37_04640)	-	422470..423822 (+)	1353	WP_014630116.1	tetratricopeptide repeat protein	-
LDO80_RS02365 (TthAA37_04650)	-	423843..424874 (-)	1032	WP_143584725.1	DUF4388 domain-containing protein	-
LDO80_RS02370 (TthAA37_04660)	ruvB	424871..425845 (-)	975	WP_011227913.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
LDO80_RS02375 (TthAA37_04670)	panB	425871..426656 (+)	786	WP_014630114.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
LDO80_RS02380 (TthAA37_04680)	-	426704..427630 (+)	927	WP_014630113.1	RluA family pseudouridine synthase	-
LDO80_RS02385 (TthAA37_04690)	-	427627..428520 (+)	894	WP_223965972.1	DNA-methyltransferase	-
LDO80_RS02390 (TthAA37_04700)	-	428495..429172 (+)	678	WP_172619159.1	hypothetical protein	-
LDO80_RS02395 (TthAA37_04710)	lysN	429158..430351 (-)	1194	WP_143584730.1	2-aminoadipate transaminase	-

Sequence

Protein

• Length: 324 a.a.
• Molecular weight: 35973.74 Da
• Isoelectric Point: 6.3232

>NTDB_id=75951 LDO80_RS02370 WP_011227913.1 424871..425845(-) (ruvB) [Thermus thermophilus strain AA3-7]
MEDLALRPKTLDEYIGQERLKQKLRVYLEAAKARKEPLEHLLLFGPPGLGKTTLAHVIAHELGVNLRVTSGPAIEKPGDL
AAILANSLEEGDILFIDEIHRLSRQAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAPLLSRFG
IVEHLEYYTPEELAQGVMRDARLLGVRITEEAALEIGRRSRGTMRVAKRLFRRVRDFAQVAGEEVITRERALEALAALGL
DELGLEKRDREILEVLILRFGGGPVGLATLATALSEDPGTLEEVHEPYLIRQGLLKRTPRGRVATELAYRHLGYPPPVGP
LLEP

Nucleotide

• Length: 975 bp

>NTDB_id=75951 LDO80_RS02370 WP_011227913.1 424871..425845(-) (ruvB) [Thermus thermophilus strain AA3-7]
GTGGAAGACCTCGCCCTTAGGCCCAAGACCCTGGACGAGTACATCGGCCAGGAACGCCTGAAGCAAAAGCTCCGGGTCTA
CCTCGAGGCGGCCAAGGCCCGAAAAGAGCCCTTGGAGCACCTCCTCCTCTTCGGCCCCCCGGGCCTGGGCAAGACCACCC
TGGCCCACGTCATCGCCCACGAGCTTGGGGTCAACCTCCGGGTCACCTCCGGGCCCGCCATAGAGAAGCCCGGGGACCTC
GCCGCCATCCTGGCCAACTCCCTGGAGGAAGGGGACATCCTCTTCATTGACGAGATCCACCGCTTGAGCCGCCAGGCCGA
GGAGCACCTCTACCCCGCCATGGAGGACTTCGTCATGGACATCGTCATCGGCCAAGGTCCGGCGGCGAGAACCATCCGGC
TGGAGCTTCCCCGCTTCACCCTGATCGGGGCCACCACCCGGCCCGGCCTCATCACCGCGCCCCTCTTGAGCCGCTTCGGC
ATCGTGGAGCACCTGGAGTACTACACCCCCGAGGAGCTGGCCCAAGGGGTGATGCGGGACGCCCGCCTCCTCGGGGTGAG
GATCACGGAGGAGGCCGCCCTGGAGATCGGTAGGCGGAGCCGGGGCACCATGCGCGTCGCCAAGCGCCTCTTCCGGAGGG
TGCGGGACTTCGCCCAGGTGGCGGGGGAGGAGGTCATCACCCGGGAGCGGGCCCTGGAGGCCCTTGCGGCCCTGGGCCTG
GACGAGCTGGGCTTGGAGAAGCGGGACCGGGAGATCCTGGAGGTCCTCATCCTCCGCTTCGGCGGTGGCCCAGTGGGCCT
CGCCACCTTAGCCACCGCCCTCTCCGAGGACCCGGGCACCTTGGAAGAGGTGCACGAGCCCTACCTCATCCGCCAGGGCC
TTTTGAAGCGCACTCCCCGGGGCCGGGTGGCCACGGAACTGGCCTACCGCCACCTGGGCTACCCGCCCCCGGTGGGCCCC
CTCTTGGAGCCGTGA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (168-241)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (6-165)

• RuvB C-terminal winged helix domain (WHD_RuvB) (242-312)

3D structure

• PDB: 1HQC
• PDB: 1IXR
• PDB: 1IXS
• PDB: 8EFV
• PDB: 8EFY

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.105	96.605	0.571
ruvB	Streptococcus pneumoniae TIGR4	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae R6	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae D39	56.774	95.679	0.543
ruvB	Synechocystis sp. PCC 6803	50.779	99.074	0.503
ruvB	Helicobacter pylori 26695	50.637	96.914	0.491