Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ORF34_RS13435 Genome accession   NZ_CP112873
Coordinates   2560810..2561880 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain O4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2555810..2566880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORF34_RS13390 (ORF34_13390) tapA 2556288..2557049 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  ORF34_RS13395 (ORF34_13395) yqzG 2557322..2557648 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  ORF34_RS13400 (ORF34_13400) spoIITA 2557690..2557869 (-) 180 WP_003230176.1 YqzE family protein -
  ORF34_RS13405 (ORF34_13405) comGG 2557940..2558314 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  ORF34_RS13410 (ORF34_13410) comGF 2558315..2558698 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  ORF34_RS13415 (ORF34_13415) comGE 2558724..2559071 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  ORF34_RS13420 (ORF34_13420) comGD 2559055..2559486 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  ORF34_RS13425 (ORF34_13425) comGC 2559476..2559772 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  ORF34_RS13430 (ORF34_13430) comGB 2559786..2560823 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  ORF34_RS13435 (ORF34_13435) comGA 2560810..2561880 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  ORF34_RS13440 (ORF34_13440) corA 2562292..2563245 (-) 954 WP_004399136.1 magnesium transporter CorA -
  ORF34_RS13445 (ORF34_13445) yqhB 2563388..2564716 (+) 1329 WP_009967730.1 hemolysin family protein -
  ORF34_RS13450 (ORF34_13450) rsbRD 2564769..2565605 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  ORF34_RS13460 (ORF34_13460) mgsR 2565829..2566209 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  ORF34_RS13465 (ORF34_13465) yqgY 2566441..2566686 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=758590 ORF34_RS13435 WP_004399124.1 2560810..2561880(-) (comGA) [Bacillus subtilis strain O4]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=758590 ORF34_RS13435 WP_004399124.1 2560810..2561880(-) (comGA) [Bacillus subtilis strain O4]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1