Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ORF34_RS13430 Genome accession   NZ_CP112873
Coordinates   2559786..2560823 (-) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus subtilis strain O4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2554786..2565823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORF34_RS13380 (ORF34_13380) tasA 2554883..2555668 (-) 786 WP_004398632.1 biofilm matrix protein TasA -
  ORF34_RS13385 (ORF34_13385) sipW 2555732..2556304 (-) 573 WP_003246088.1 signal peptidase I SipW -
  ORF34_RS13390 (ORF34_13390) tapA 2556288..2557049 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  ORF34_RS13395 (ORF34_13395) yqzG 2557322..2557648 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  ORF34_RS13400 (ORF34_13400) spoIITA 2557690..2557869 (-) 180 WP_003230176.1 YqzE family protein -
  ORF34_RS13405 (ORF34_13405) comGG 2557940..2558314 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  ORF34_RS13410 (ORF34_13410) comGF 2558315..2558698 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  ORF34_RS13415 (ORF34_13415) comGE 2558724..2559071 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  ORF34_RS13420 (ORF34_13420) comGD 2559055..2559486 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  ORF34_RS13425 (ORF34_13425) comGC 2559476..2559772 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  ORF34_RS13430 (ORF34_13430) comGB 2559786..2560823 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  ORF34_RS13435 (ORF34_13435) comGA 2560810..2561880 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  ORF34_RS13440 (ORF34_13440) corA 2562292..2563245 (-) 954 WP_004399136.1 magnesium transporter CorA -
  ORF34_RS13445 (ORF34_13445) yqhB 2563388..2564716 (+) 1329 WP_009967730.1 hemolysin family protein -
  ORF34_RS13450 (ORF34_13450) rsbRD 2564769..2565605 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=758589 ORF34_RS13430 WP_009967727.1 2559786..2560823(-) (comGB) [Bacillus subtilis strain O4]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=758589 ORF34_RS13430 WP_009967727.1 2559786..2560823(-) (comGB) [Bacillus subtilis strain O4]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936