NTDB

Overview

Name	pilU	Type	Machinery gene
Locus tag	OPM66_RS04380	Genome accession	NZ_CP112860
Coordinates	925143..926261 (-)	Length	372 a.a.
NCBI ID	WP_000347039.1	Uniprot ID	A0A9P2XLL8
Organism	Acinetobacter baumannii strain EAB2
Function	mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 920143..931261

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OPM66_RS04350	-	920972..921820 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
OPM66_RS04355	-	922073..922528 (+)	456	WP_000782976.1	bacteriohemerythrin	-
OPM66_RS04360	-	922681..923748 (+)	1068	WP_002019572.1	hypothetical protein	-
OPM66_RS04365	-	923748..924071 (+)	324	WP_000442587.1	RnfH family protein	-
OPM66_RS04370	-	924126..924524 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OPM66_RS04375	fur	924636..925073 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OPM66_RS04380	pilU	925143..926261 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OPM66_RS04385	pilT	926289..927326 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OPM66_RS04390	-	927453..928145 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

Download Length: 372 a.a. Molecular weight: 41933.13 Da Isoelectric Point: 6.6944

                                >NTDB_id=758399 OPM66_RS04380 WP_000347039.1 925143..926261(-) (pilU) [Acinetobacter baumannii strain EAB2]

MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA

RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE

DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR

IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ

ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

Download Length: 1119 bp

                                >NTDB_id=758399 OPM66_RS04380 WP_000347039.1 925143..926261(-) (pilU) [Acinetobacter baumannii strain EAB2]

ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC

TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA

TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG

CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT

TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG

GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA

GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA

AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT

ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT

ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA

GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT

ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA

GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG

TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395

Detailed information NTG0758399