Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OPS05_RS26375 Genome accession   NZ_CP111114
Coordinates   5828493..5829527 (-) Length   344 a.a.
NCBI ID   WP_003229114.1    Uniprot ID   A0A031GC41
Organism   Pseudomonas koreensis strain FP1691     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5823493..5834527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPS05_RS26345 (OPS05_26345) ruvX 5823575..5824012 (+) 438 WP_003229101.1 Holliday junction resolvase RuvX -
  OPS05_RS26350 (OPS05_26350) pyrR 5824094..5824600 (+) 507 WP_027610583.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OPS05_RS26355 (OPS05_26355) - 5824629..5825633 (+) 1005 WP_003229107.1 aspartate carbamoyltransferase catalytic subunit -
  OPS05_RS26360 (OPS05_26360) - 5825630..5826901 (+) 1272 WP_098966064.1 dihydroorotase -
  OPS05_RS26365 (OPS05_26365) - 5827054..5827485 (-) 432 WP_007964113.1 NINE protein -
  OPS05_RS26370 (OPS05_26370) - 5827734..5828360 (+) 627 WP_003229112.1 C40 family peptidase -
  OPS05_RS26375 (OPS05_26375) pilT 5828493..5829527 (-) 1035 WP_003229114.1 type IV pilus twitching motility protein PilT Machinery gene
  OPS05_RS26380 (OPS05_26380) - 5829584..5830270 (+) 687 WP_134784871.1 YggS family pyridoxal phosphate-dependent enzyme -
  OPS05_RS26385 (OPS05_26385) proC 5830299..5831117 (+) 819 WP_098966067.1 pyrroline-5-carboxylate reductase -
  OPS05_RS26390 (OPS05_26390) - 5831128..5831718 (+) 591 WP_027610578.1 YggT family protein -
  OPS05_RS26395 (OPS05_26395) - 5831715..5832011 (+) 297 WP_134784872.1 DUF167 domain-containing protein -
  OPS05_RS26400 (OPS05_26400) metX 5832240..5833379 (+) 1140 WP_134784873.1 homoserine O-succinyltransferase MetX -
  OPS05_RS26405 (OPS05_26405) metW 5833387..5834007 (+) 621 WP_003229126.1 methionine biosynthesis protein MetW -
  OPS05_RS26410 (OPS05_26410) - 5834034..5834468 (+) 435 WP_027610575.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0158

>NTDB_id=757962 OPS05_RS26375 WP_003229114.1 5828493..5829527(-) (pilT) [Pseudomonas koreensis strain FP1691]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KELVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=757962 OPS05_RS26375 WP_003229114.1 5828493..5829527(-) (pilT) [Pseudomonas koreensis strain FP1691]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACACCTCGAAACGGATTTCTCGTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAACGCGTTCAACCAGAACCGGGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGAAAGTGCTGAGCATGGA
AGACCTCGGCATGGGCGATGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGGT
CCGGCAAATCCACCACGTTGGCGGCGATGATCGATTACCTCAACACCCATCGTCACCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGCTTCGCCAC
GGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACGTGATTCTGGTTGGCGAGATGCGCGACCTGGAAACCATTCGCCTCG
CACTGACTGCCGCTGAGACCGGGCATCTGGTGTTCGGCACGCTGCACACGACGTCCGCCGCCAAGACCATCGACCGTGTT
GTCGATGTGTTCCCCGGGGACGAGAAGTCCATGGTCCGCTCGATGCTTTCGGAGTCGTTGCTGGCCGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGGCGGGTCGCGGCCCACGAGATCATGCTGGGAACGTCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAAATGTATTCGGCGATTCAGACCGGAGGGTCGTTGGGGATGCAGACACTCGACATGTGCCTG
AAGGAGCTGGTGACCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGAAAAGGCGCGTACGCCGGATAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A031GC41

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398