Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   F7G76_RS11745 Genome accession   NZ_AP024924
Coordinates   2344775..2345785 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli O145:H28 strain 16003     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2339775..2350785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7G76_RS11725 (EC16003_2258) mepM 2340643..2341965 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  F7G76_RS11730 (EC16003_2259) znuA 2341981..2342913 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  F7G76_RS11735 (EC16003_2260) znuC 2342992..2343747 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  F7G76_RS11740 (EC16003_2261) znuB 2343744..2344529 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  F7G76_RS11745 (EC16003_2262) ruvB 2344775..2345785 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  F7G76_RS11750 (EC16003_2263) ruvA 2345794..2346405 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  F7G76_RS11755 yobI 2346544..2346609 (-) 66 WP_072095795.1 stress response small protein YobI -
  F7G76_RS11760 (EC16003_2264) yebB 2346680..2347282 (+) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  F7G76_RS11765 (EC16003_2265) ruvC 2347284..2347805 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  F7G76_RS11770 (EC16003_2266) yebC 2347840..2348580 (-) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  F7G76_RS11775 (EC16003_2267) nudB 2348609..2349061 (-) 453 WP_032161923.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=75769 F7G76_RS11745 WP_000568519.1 2344775..2345785(-) (ruvB) [Escherichia coli O145:H28 strain 16003]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=75769 F7G76_RS11745 WP_000568519.1 2344775..2345785(-) (ruvB) [Escherichia coli O145:H28 strain 16003]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACTTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment