Detailed information NTG0757508

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA50_RS02195
• Genome accession: NZ_CP111034
• Coordinates: 467387..468535 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA50
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 462387..473535

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA50_RS02170	-	463538..463834 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA50_RS02175 (PALA50_00431)	-	463831..464424 (-)	594	WP_003084544.1	YggT family protein	-
PALA50_RS02180 (PALA50_00432)	proC	464435..465256 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA50_RS02185 (PALA50_00433)	-	465268..465960 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA50_RS02190 (PALA50_00434)	pilT	466175..467209 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA50_RS02195 (PALA50_00435)	pilU	467387..468535 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA50_RS02200 (PALA50_00436)	czcD	468543..469442 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA50_RS02205 (PALA50_00437)	-	469596..470000 (+)	405	WP_003084560.1	NINE protein	-
PALA50_RS02210 (PALA50_00438)	-	470243..471616 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA50_RS02215 (PALA50_00439)	-	471613..472797 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=757508 PALA50_RS02195 WP_003100959.1 467387..468535(+) (pilU) [Pseudomonas aeruginosa strain PALA50]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=757508 PALA50_RS02195 WP_003100959.1 467387..468535(+) (pilU) [Pseudomonas aeruginosa strain PALA50]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATTGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382