Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   OPU65_RS00120 Genome accession   NZ_CP110812
Coordinates   17682..18311 (+) Length   209 a.a.
NCBI ID   WP_301441141.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CamBx3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12682..23311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPU65_RS00085 (OPU65_00085) aroE 13324..14160 (+) 837 WP_243943036.1 shikimate dehydrogenase -
  OPU65_RS00090 (OPU65_00090) yhbY 14154..14444 (+) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  OPU65_RS00095 (OPU65_00095) - 14463..15032 (+) 570 WP_243943038.1 nicotinate-nucleotide adenylyltransferase -
  OPU65_RS00100 (OPU65_00100) yqeK 15022..15585 (+) 564 WP_243943039.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  OPU65_RS00105 (OPU65_00105) rsfS 15599..15955 (+) 357 WP_059231259.1 ribosome silencing factor -
  OPU65_RS00110 (OPU65_00110) - 15952..16707 (+) 756 WP_265626256.1 class I SAM-dependent methyltransferase -
  OPU65_RS00115 (OPU65_00115) comER 16778..17599 (-) 822 WP_265626257.1 late competence protein ComER -
  OPU65_RS00120 (OPU65_00120) comEA 17682..18311 (+) 630 WP_301441141.1 helix-hairpin-helix domain-containing protein Machinery gene
  OPU65_RS00125 (OPU65_00125) - 18380..18949 (+) 570 WP_243943042.1 ComE operon protein 2 -
  OPU65_RS00130 (OPU65_00130) - 18954..21341 (+) 2388 WP_265626259.1 DNA internalization-related competence protein ComEC/Rec2 -
  OPU65_RS00135 (OPU65_00135) - 21365..21496 (-) 132 WP_003183686.1 YqzM family protein -
  OPU65_RS00140 (OPU65_00140) holA 21726..22751 (+) 1026 WP_265626262.1 DNA polymerase III subunit delta -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22684.55 Da        Isoelectric Point: 4.7360

>NTDB_id=756331 OPU65_RS00120 WP_301441141.1 17682..18311(+) (comEA) [Bacillus paralicheniformis strain CamBx3]
MTDWLKQYKWHAAGSVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTFEKKEEVKREKNAGREEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADEKQINLAAVLRDGMVLYIPFEGEEAAGSFSEADSRADGSSGDIVNINTASSEELQT
IPGIGPSKAEAVIEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=756331 OPU65_RS00120 WP_301441141.1 17682..18311(+) (comEA) [Bacillus paralicheniformis strain CamBx3]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAAGCGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCAGGCCTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGAGAAGAAAG
AGGAGGTGAAGCGGGAAAAAAACGCGGGGAGAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACGAAAAGCA
AATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCTGCTGGCTCTTTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAGGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAATCGAATACCGCGAGGAAAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTTCAATCACAGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(146-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.885

99.522

0.526
  comEA Staphylococcus aureus MW2

35.455

100

0.373
  comEA Staphylococcus aureus N315

35.455

100

0.373
  comEA Lactococcus lactis subsp. cremoris KW2

35.484

100

0.368