Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LFL98_RS12955 Genome accession   NZ_CP110365
Coordinates   2499772..2500389 (-) Length   205 a.a.
NCBI ID   WP_032722140.1    Uniprot ID   -
Organism   Bacillus subtilis strain HY2-62     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2494772..2505389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFL98_RS12925 (LFL98_12930) rpsT 2495074..2495340 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  LFL98_RS12930 (LFL98_12935) holA 2495355..2496398 (-) 1044 WP_277736541.1 DNA polymerase III subunit delta -
  LFL98_RS12935 (LFL98_12940) - 2496438..2496587 (-) 150 WP_003229985.1 hypothetical protein -
  LFL98_RS12940 (LFL98_12945) yqzM 2496628..2496762 (+) 135 WP_003229983.1 YqzM family protein -
  LFL98_RS12945 (LFL98_12950) comEC 2496802..2499132 (-) 2331 WP_277736542.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LFL98_RS12950 (LFL98_12955) comEB 2499136..2499705 (-) 570 WP_003229978.1 ComE operon protein 2 -
  LFL98_RS12955 (LFL98_12960) comEA 2499772..2500389 (-) 618 WP_032722140.1 competence protein ComEA Machinery gene
  LFL98_RS12960 (LFL98_12965) comER 2500473..2501294 (+) 822 WP_032722142.1 late competence protein ComER -
  LFL98_RS12965 (LFL98_12970) yqeM 2501361..2502104 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  LFL98_RS12970 (LFL98_12975) rsfS 2502101..2502457 (-) 357 WP_041333759.1 ribosome silencing factor -
  LFL98_RS12975 (LFL98_12980) yqeK 2502475..2503035 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LFL98_RS12980 (LFL98_12985) nadD 2503025..2503594 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  LFL98_RS12985 (LFL98_12990) yhbY 2503606..2503896 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  LFL98_RS12990 (LFL98_12995) aroE 2503890..2504732 (-) 843 WP_076458133.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21748.46 Da        Isoelectric Point: 4.8354

>NTDB_id=754635 LFL98_RS12955 WP_032722140.1 2499772..2500389(-) (comEA) [Bacillus subtilis strain HY2-62]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVRHPGVYELRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=754635 LFL98_RS12955 WP_032722140.1 2499772..2500389(-) (comEA) [Bacillus subtilis strain HY2-62]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAATTGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38