Detailed information NTG0754152

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA24_RS02035
• Genome accession: NZ_CP110344
• Coordinates: 439114..440262 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA24
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 434114..445262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA24_RS02010	-	435265..435561 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA24_RS02015 (PALA24_00403)	-	435558..436151 (-)	594	WP_003084544.1	YggT family protein	-
PALA24_RS02020 (PALA24_00404)	proC	436162..436983 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA24_RS02025 (PALA24_00405)	-	436995..437687 (-)	693	WP_003118803.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA24_RS02030 (PALA24_00406)	pilT	437902..438936 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA24_RS02035 (PALA24_00407)	pilU	439114..440262 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA24_RS02040 (PALA24_00408)	czcD	440270..441169 (-)	900	WP_003118804.1	CDF family zinc transporter CzcD	-
PALA24_RS02045 (PALA24_00409)	-	441323..441727 (+)	405	WP_003084560.1	NINE protein	-
PALA24_RS02050 (PALA24_00410)	-	441970..443343 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA24_RS02055 (PALA24_00411)	-	443340..444524 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=754152 PALA24_RS02035 WP_003100959.1 439114..440262(+) (pilU) [Pseudomonas aeruginosa strain PALA24]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=754152 PALA24_RS02035 WP_003100959.1 439114..440262(+) (pilU) [Pseudomonas aeruginosa strain PALA24]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGTATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGTAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382