Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OMP39_RS02340 Genome accession   NZ_CP110257
Coordinates   515435..517150 (-) Length   571 a.a.
NCBI ID   WP_264893206.1    Uniprot ID   -
Organism   Schlegelella aquatica strain LMG 23380     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 510435..522150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OMP39_RS02310 (OMP39_02310) - 511071..511523 (+) 453 WP_264893200.1 NUDIX domain-containing protein -
  OMP39_RS02315 (OMP39_02315) - 511612..511848 (-) 237 WP_264893201.1 DNA gyrase inhibitor YacG -
  OMP39_RS02320 (OMP39_02320) zapD 511850..512602 (-) 753 WP_264893202.1 cell division protein ZapD -
  OMP39_RS02325 (OMP39_02325) coaE 512771..513373 (-) 603 WP_264893203.1 dephospho-CoA kinase -
  OMP39_RS02330 (OMP39_02330) pilD 513436..514293 (-) 858 WP_264893204.1 A24 family peptidase Machinery gene
  OMP39_RS02335 (OMP39_02335) pilC 514300..515394 (-) 1095 WP_264893205.1 type II secretion system F family protein Machinery gene
  OMP39_RS02340 (OMP39_02340) pilB 515435..517150 (-) 1716 WP_264893206.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OMP39_RS02345 (OMP39_02345) recD 517348..519186 (-) 1839 WP_264893207.1 exodeoxyribonuclease V subunit alpha -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62252.55 Da        Isoelectric Point: 6.0098

>NTDB_id=753412 OMP39_RS02340 WP_264893206.1 515435..517150(-) (pilB) [Schlegelella aquatica strain LMG 23380]
MDTLAEAPQSTLTGVARVLVNAGKLNARTAEELAKTAQDKRISFISAVIAAGAVSASDLAHALSHAFSLPLVDLNAIDLQ
RLPRGVVDNKLAAQYQVVVLGKRGSRLFVGGADPTNQEALERIKFATQLTPEWIIVEHDKLTKLLETMGTSAAEALESIA
GGDFEFDVSEEDATQEAADVSTDVEDAPVVRFLQKMLIDAINMRASDLHFEPYEYNYRVRFRIDGELREIAQPPIAIKDK
LASRIKVISKLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEKEEKERLLRAIE
RPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTVEDPAEINLPGINQVNVNDKAGLTFAAALKAFLRQDPDIIMVGE
IRDLETADIAIKAAQTGHLVMSTLHTNDAPTTLTRLRNMGVAPFNIASSVILITAQRLARRLCENCKTPAVYPREALLRA
GFKEEDLDGSWKPYRAVGCSSCNNGYKGRVGIYQVMPISEEIQRIILADGSAMDIAAQAQKEGVRDLRQAGLHKVKLGVT
SLEEVISVTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=753412 OMP39_RS02340 WP_264893206.1 515435..517150(-) (pilB) [Schlegelella aquatica strain LMG 23380]
ATGGACACACTCGCTGAGGCTCCCCAAAGTACCCTGACCGGGGTGGCGCGGGTACTGGTCAACGCGGGCAAGCTGAACGC
CCGCACCGCCGAAGAGCTCGCCAAGACCGCGCAGGACAAGCGCATCAGCTTCATCTCTGCCGTCATCGCGGCCGGCGCGG
TCTCGGCCAGCGACTTGGCCCACGCCCTTTCGCACGCGTTCTCGCTCCCGCTGGTCGATCTCAATGCGATCGACCTGCAG
CGGCTGCCCCGGGGCGTCGTCGACAACAAGCTGGCCGCGCAGTACCAGGTGGTGGTGCTCGGCAAACGCGGCAGCCGGCT
GTTCGTCGGCGGGGCCGACCCCACCAACCAGGAAGCGCTGGAGCGCATCAAGTTCGCCACCCAGCTCACGCCCGAATGGA
TCATCGTCGAGCACGACAAGCTCACCAAGCTGCTCGAGACGATGGGCACGAGCGCGGCGGAGGCCCTCGAGAGCATCGCC
GGCGGGGACTTCGAGTTCGACGTCTCCGAGGAGGACGCCACCCAGGAAGCGGCCGACGTCTCCACCGACGTCGAAGACGC
CCCGGTGGTGCGCTTCCTGCAGAAGATGCTGATCGACGCGATCAACATGCGCGCGTCCGACCTGCACTTCGAGCCCTACG
AATACAACTACCGGGTGCGCTTCCGCATCGACGGCGAACTGCGCGAGATCGCCCAGCCGCCGATCGCCATCAAGGACAAG
CTCGCCTCGCGCATCAAGGTCATCTCCAAGCTCGACATCGCCGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTGAA
GTTCGGCAGCAAGGCGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTCATCCGTATCCTGG
ACCCGTCGTCGGCCAAGCTCGGCATCGACGCGCTGGGCTACGAGAAGGAGGAAAAAGAGCGCCTGCTGCGCGCCATCGAG
CGGCCGTACGGGATGATCCTGGTCACCGGCCCCACCGGCAGCGGCAAGACGGTCTCGCTCTACACCTGCCTGAACCTGCT
CAACAAGCCGGGCGTCAACATCTCCACCGTCGAAGATCCGGCGGAAATCAACCTGCCCGGCATCAACCAGGTCAACGTCA
ACGACAAGGCGGGTCTGACCTTCGCCGCCGCGCTCAAGGCCTTTCTGCGACAGGACCCGGACATCATCATGGTCGGTGAG
ATCCGCGACCTGGAGACGGCCGACATCGCGATCAAGGCGGCGCAGACGGGTCACCTGGTGATGTCCACGCTGCACACCAA
CGACGCGCCGACGACGCTCACGCGGCTGCGCAACATGGGCGTCGCACCGTTCAACATCGCGTCCAGCGTGATCCTCATCA
CCGCCCAGCGCCTGGCGCGCCGCCTGTGCGAGAACTGCAAGACGCCCGCGGTGTACCCGCGCGAGGCCCTGCTGCGCGCG
GGCTTCAAGGAAGAGGATCTGGACGGCAGCTGGAAGCCGTACCGCGCCGTGGGCTGCTCCAGCTGCAACAATGGCTACAA
GGGCCGCGTCGGCATCTACCAGGTCATGCCCATCTCGGAGGAGATCCAACGCATCATCCTGGCCGACGGCAGCGCGATGG
ACATCGCGGCGCAGGCCCAGAAGGAGGGCGTGCGGGACTTGCGACAGGCGGGCCTGCACAAGGTCAAGCTCGGCGTCACC
TCGCTCGAGGAAGTCATCTCGGTCACGAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(195-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.556

100

0.56
  pilB Acinetobacter baylyi ADP1

55.263

99.825

0.552
  pilF Neisseria gonorrhoeae MS11

54.804

98.424

0.539
  pilB Legionella pneumophila strain ERS1305867

50.88

99.475

0.506
  pilB Vibrio cholerae strain A1552

47.679

98.074

0.468
  pilB Vibrio campbellii strain DS40M4

46.702

98.249

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

46.099

98.774

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.678

92.995

0.378
  pilF Thermus thermophilus HB27

38.23

98.949

0.378