Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D3P44_RS19340 Genome accession   NZ_CP110110
Coordinates   4075216..4076361 (-) Length   381 a.a.
NCBI ID   WP_041108398.1    Uniprot ID   A0A8D3Y4G4
Organism   Stutzerimonas balearica strain Z8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4070216..4081361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3P44_RS19315 (D3P44_019315) - 4070332..4071354 (+) 1023 WP_268845828.1 aspartate carbamoyltransferase catalytic subunit -
  D3P44_RS19320 (D3P44_019320) - 4071348..4072619 (+) 1272 WP_061339830.1 dihydroorotase -
  D3P44_RS19325 (D3P44_019325) - 4072657..4073058 (-) 402 WP_041108393.1 NINE protein -
  D3P44_RS19330 (D3P44_019330) - 4073155..4074366 (+) 1212 WP_212543505.1 putative DNA modification/repair radical SAM protein -
  D3P44_RS19335 (D3P44_019335) - 4074366..4075250 (+) 885 WP_212543504.1 TIGR03915 family putative DNA repair protein -
  D3P44_RS19340 (D3P44_019340) pilU 4075216..4076361 (-) 1146 WP_041108398.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D3P44_RS19345 (D3P44_019345) pilT 4076438..4077472 (-) 1035 WP_043222415.1 type IV pilus twitching motility protein PilT Machinery gene
  D3P44_RS19350 (D3P44_019350) - 4077538..4078227 (+) 690 WP_212543503.1 YggS family pyridoxal phosphate-dependent enzyme -
  D3P44_RS19355 (D3P44_019355) proC 4078247..4079080 (+) 834 WP_061339828.1 pyrroline-5-carboxylate reductase -
  D3P44_RS19360 (D3P44_019360) - 4079090..4079680 (+) 591 WP_041108406.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42517.82 Da        Isoelectric Point: 6.6647

>NTDB_id=752430 D3P44_RS19340 WP_041108398.1 4075216..4076361(-) (pilU) [Stutzerimonas balearica strain Z8]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPDVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNTSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYVQGEITYEDALAHADSANDLRLMIKLGSETDSEHLTSVSQGLRLEVSDDDPGFRMR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=752430 D3P44_RS19340 WP_041108398.1 4075216..4076361(-) (pilU) [Stutzerimonas balearica strain Z8]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAGAAGGGCGGCTCCGACCTGTTCATCACGGCCGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGCCGATGTCGCCGGAGATGACCCGCGAAACCGTGCATGGGG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAGAACCACGAGTGCAACTTCGCCATCAGCGCTCGGGGGATCGGGCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAACCTCGCCGGCATGGTCCTGCGGCGGATCGAGACCAACATTCCCACGCTCGA
AGACCTCAAACTGCCCGACGTGCTGAAGAAGCTGGCGCTGACCAAGCGGGGGCTGGTGCTGTTCGTCGGGGCGACCGGTA
CTGGCAAATCCACGTCGCTGGCGGCAATGATTGGCTACCGCAACAAGAACACCAGTGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAGCTGCATCGTGACTCAGCGCGAGGTGGGCATCGATACGGAGTCGTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCTGACGTCATCCTCATCGGCGAAGTGCGGACCCGCGAGACGATGGATTATG
CCGTGGCGTTCGCCGAGACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCTGATCGGCAGCAGCAGGTATGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACACCCGACGGCAAGGGACGGCGAGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGGGGCTGATGAAGCGATCCACCGAGCAAGGCATGCAGACCTTCGATCAGGCG
CTCTACAACCTCTATGTGCAAGGCGAGATCACCTACGAGGACGCCTTGGCTCATGCCGATTCGGCCAACGATCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGATAGCGAGCACCTCACCAGCGTGTCCCAGGGGCTGCGCCTGGAGGTCAGTGACG
ACGATCCGGGCTTCCGGATGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

95.276

100

0.953
  pilU Acinetobacter baylyi ADP1

65.546

93.701

0.614
  pilU Vibrio cholerae strain A1552

60

91.864

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

88.976

0.386
  pilT Legionella pneumophila strain Lp02

41.493

87.927

0.365
  pilT Legionella pneumophila strain ERS1305867

41.493

87.927

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.124

88.714

0.365
  pilT Vibrio cholerae strain A1552

41.124

88.714

0.365
  pilT Neisseria gonorrhoeae MS11

40

90.551

0.362
  pilT Neisseria meningitidis 8013

40

90.551

0.362