Detailed information NTG0751228

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: QFB77_RS11555
• Genome accession: NZ_CP123592
• Coordinates: 2029663..2030673 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli strain DJH_137urine
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2024663..2035673

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QFB77_RS11525	nudB	2026387..2026839 (+)	453	WP_001443602.1	dihydroneopterin triphosphate diphosphatase	-
QFB77_RS11530	yebC	2026868..2027608 (+)	741	WP_000907248.1	YebC/PmpR family DNA-binding transcriptional regulator	-
QFB77_RS11535	ruvC	2027643..2028164 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
QFB77_RS11540	yebB	2028166..2028768 (-)	603	WP_001024930.1	YebB family permuted papain-like enzyme	-
QFB77_RS11545	yobI	2028839..2028904 (+)	66	WP_001386853.1	stress response small protein YobI	-
QFB77_RS11550	ruvA	2029043..2029654 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
QFB77_RS11555	ruvB	2029663..2030673 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
QFB77_RS11560	znuB	2030820..2031605 (-)	786	WP_000571479.1	zinc ABC transporter permease subunit ZnuB	-
QFB77_RS11565	znuC	2031602..2032357 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
QFB77_RS11570	znuA	2032436..2033368 (+)	933	WP_001342995.1	zinc ABC transporter substrate-binding protein ZnuA	-
QFB77_RS11575	mepM	2033384..2034706 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=751228 QFB77_RS11555 WP_000568519.1 2029663..2030673(+) (ruvB) [Escherichia coli strain DJH_137urine]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=751228 QFB77_RS11555 WP_000568519.1 2029663..2030673(+) (ruvB) [Escherichia coli strain DJH_137urine]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494