Detailed information NTG0750455

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OK116_RS06400
• Genome accession: NZ_CP109897
• Coordinates: 1354646..1355776 (+)
• Length: 376 a.a.
• NCBI ID: WP_118853444.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. fastidiosa strain XYL461
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1349646..1360776

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OK116_RS06380 (OK116_06375)	-	1349933..1350757 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
OK116_RS06385 (OK116_06380)	-	1351391..1352218 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
OK116_RS06390 (OK116_06385)	-	1352576..1352788 (-)	213	WP_004087301.1	hypothetical protein	-
OK116_RS06395 (OK116_06390)	pilT	1353446..1354480 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
OK116_RS06400 (OK116_06395)	pilU	1354646..1355776 (+)	1131	WP_118853444.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OK116_RS06405 (OK116_06400)	-	1356469..1357437 (-)	969	WP_004087304.1	YafY family protein	-
OK116_RS06410 (OK116_06405)	-	1358286..1359095 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
OK116_RS06415 (OK116_06410)	hemC	1359149..1360066 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42410.62 Da
• Isoelectric Point: 6.4489

>NTDB_id=750455 OK116_RS06400 WP_118853444.1 1354646..1355776(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain XYL461]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLSQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=750455 OK116_RS06400 WP_118853444.1 1354646..1355776(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain XYL461]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTAAGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.308	97.606	0.598
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	50.562	94.681	0.479
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.436	96.277	0.399