Detailed information NTG0750438

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OK118_RS06185
• Genome accession: NZ_CP109894
• Coordinates: 1354854..1355984 (+)
• Length: 376 a.a.
• NCBI ID: WP_128712541.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. fastidiosa strain CFBP8351
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1349854..1360984

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OK118_RS06165 (OK118_06165)	-	1350141..1350965 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
OK118_RS06170 (OK118_06170)	-	1351599..1352426 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
OK118_RS06175 (OK118_06175)	-	1352784..1352996 (-)	213	WP_004087301.1	hypothetical protein	-
OK118_RS06180 (OK118_06180)	pilT	1353654..1354688 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
OK118_RS06185 (OK118_06185)	pilU	1354854..1355984 (+)	1131	WP_128712541.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OK118_RS06190 (OK118_06190)	-	1356677..1357645 (-)	969	WP_004087304.1	YafY family protein	-
OK118_RS06195 (OK118_06195)	-	1358494..1359303 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
OK118_RS06200 (OK118_06200)	hemC	1359357..1360274 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42525.82 Da
• Isoelectric Point: 6.5550

>NTDB_id=750438 OK118_RS06185 WP_128712541.1 1354854..1355984(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain CFBP8351]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHCNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=750438 OK118_RS06185 WP_128712541.1 1354854..1355984(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain CFBP8351]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACTGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.308	97.606	0.598
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	50.562	94.681	0.479
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399