Detailed information NTG0750385

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OK117_RS06225
• Genome accession: NZ_CP109886
• Coordinates: 1378711..1379841 (+)
• Length: 376 a.a.
• NCBI ID: WP_011097971.1
• Uniprot ID: Q87CD3
• Organism: Xylella fastidiosa subsp. fastidiosa strain CFBP8073
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1373711..1384841

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OK117_RS06205 (OK117_06220)	-	1373979..1374803 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
OK117_RS06210 (OK117_06225)	-	1375457..1376284 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
OK117_RS06215 (OK117_06230)	-	1376642..1376854 (-)	213	WP_058564881.1	hypothetical protein	-
OK117_RS06220 (OK117_06235)	pilT	1377511..1378545 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
OK117_RS06225 (OK117_06240)	pilU	1378711..1379841 (+)	1131	WP_011097971.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OK117_RS06230 (OK117_06245)	-	1380534..1381502 (-)	969	WP_058564887.1	YafY family protein	-
OK117_RS06235 (OK117_06250)	-	1381884..1382015 (+)	132	WP_267885700.1	hypothetical protein	-
OK117_RS06240 (OK117_06255)	-	1382335..1383144 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
OK117_RS06245 (OK117_06260)	hemC	1383198..1384115 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42479.73 Da
• Isoelectric Point: 6.5563

>NTDB_id=750385 OK117_RS06225 WP_011097971.1 1378711..1379841(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain CFBP8073]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=750385 OK117_RS06225 WP_011097971.1 1378711..1379841(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain CFBP8073]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAACGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

3D structure

• AlphaFold DB: Q87CD3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.843	94.681	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402