Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QEO87_RS10010 Genome accession   NZ_CP123310
Coordinates   2073110..2074120 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain AREA_C483     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2068110..2079120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEO87_RS09980 (QEO87_09970) nudB 2069834..2070286 (+) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -
  QEO87_RS09985 (QEO87_09975) yebC 2070315..2071055 (+) 741 WP_000907251.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QEO87_RS09990 (QEO87_09980) ruvC 2071090..2071611 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  QEO87_RS09995 (QEO87_09985) yebB 2071613..2072215 (-) 603 WP_001024916.1 YebB family permuted papain-like enzyme -
  QEO87_RS10000 (QEO87_09990) yobI 2072286..2072351 (+) 66 WP_001386853.1 stress response small protein YobI -
  QEO87_RS10005 (QEO87_09995) ruvA 2072490..2073101 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  QEO87_RS10010 (QEO87_10000) ruvB 2073110..2074120 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QEO87_RS10015 (QEO87_10005) znuB 2074267..2075052 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  QEO87_RS10020 (QEO87_10010) znuC 2075049..2075804 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  QEO87_RS10025 (QEO87_10015) znuA 2075883..2076815 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  QEO87_RS10030 (QEO87_10020) mepM 2076831..2078153 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=750119 QEO87_RS10010 WP_000568519.1 2073110..2074120(+) (ruvB) [Escherichia coli strain AREA_C483]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=750119 QEO87_RS10010 WP_000568519.1 2073110..2074120(+) (ruvB) [Escherichia coli strain AREA_C483]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCGGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGTGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGTGCGCTGGAAGTAGCTC
GTCGCGCTCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAAGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494