Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FQS62_RS05070 Genome accession   NZ_CP109812
Coordinates   1081340..1082503 (+) Length   387 a.a.
NCBI ID   WP_049434422.1    Uniprot ID   A0AA43BPT5
Organism   Stenotrophomonas sp. SBJS02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1076340..1087503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FQS62_RS05040 (FQS62_005040) soxR 1076535..1076969 (+) 435 WP_099589411.1 redox-sensitive transcriptional activator SoxR -
  FQS62_RS05045 (FQS62_005045) - 1076982..1077584 (-) 603 WP_049444162.1 short chain dehydrogenase -
  FQS62_RS05050 (FQS62_005050) - 1077683..1078558 (+) 876 WP_049444163.1 LysR family transcriptional regulator -
  FQS62_RS05055 (FQS62_005055) proC 1078618..1079439 (-) 822 WP_049444164.1 pyrroline-5-carboxylate reductase -
  FQS62_RS05060 (FQS62_005060) - 1079458..1080135 (-) 678 WP_208043966.1 YggS family pyridoxal phosphate-dependent enzyme -
  FQS62_RS05065 (FQS62_005065) pilT 1080223..1081260 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  FQS62_RS05070 (FQS62_005070) pilU 1081340..1082503 (+) 1164 WP_049434422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FQS62_RS05075 (FQS62_005075) - 1082711..1083694 (+) 984 WP_049444166.1 aldo/keto reductase -
  FQS62_RS05080 (FQS62_005080) - 1083773..1084672 (-) 900 WP_226053630.1 LysR family transcriptional regulator -
  FQS62_RS05085 (FQS62_005085) - 1084999..1085211 (+) 213 WP_129550566.1 DUF4287 domain-containing protein -
  FQS62_RS05090 (FQS62_005090) - 1085322..1086029 (+) 708 WP_226053632.1 YitT family protein -
  FQS62_RS05095 (FQS62_005095) - 1086063..1086605 (-) 543 WP_208044177.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43186.63 Da        Isoelectric Point: 6.6615

>NTDB_id=749525 FQS62_RS05070 WP_049434422.1 1081340..1082503(+) (pilU) [Stenotrophomonas sp. SBJS02]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=749525 FQS62_RS05070 WP_049434422.1 1081340..1082503(+) (pilU) [Stenotrophomonas sp. SBJS02]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAACTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACCGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACCCAGA
CCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAATGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATCCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGCACCGGTAAATCGACGTCGCTGGCGGCGATGATCGGCTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATTGAATTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAAGTCGGCATTGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAAGTGCGTACCCGCGAAGGCATGGATCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCGAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCT
GATGGACCTTTCGCTGAATCTGAAGGGCGTGGTTGCGCAGCAGCTGATTCCTTCGCCCGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATTCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGTTACGA
GGACGCGCTGCGCTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGTACCC
TGTCGCAGGGGCTGGACGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

94.832

0.641
  pilU Acinetobacter baylyi ADP1

63.866

92.248

0.589
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395