Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   PAE60_RS08455 Genome accession   NZ_CP123197
Coordinates   1517411..1518421 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain C-SRM-3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1512411..1523421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAE60_RS08425 (PAE60_008425) nudB 1514135..1514587 (+) 453 WP_001443602.1 dihydroneopterin triphosphate diphosphatase -
  PAE60_RS08430 (PAE60_008430) yebC 1514616..1515356 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  PAE60_RS08435 (PAE60_008435) ruvC 1515391..1515912 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  PAE60_RS08440 (PAE60_008440) yebB 1515914..1516516 (-) 603 WP_001024930.1 YebB family permuted papain-like enzyme -
  PAE60_RS08445 (PAE60_008445) yobI 1516587..1516652 (+) 66 WP_001386853.1 stress response small protein YobI -
  PAE60_RS08450 (PAE60_008450) ruvA 1516791..1517402 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  PAE60_RS08455 (PAE60_008455) ruvB 1517411..1518421 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  PAE60_RS08460 (PAE60_008460) znuB 1518568..1519353 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  PAE60_RS08465 (PAE60_008465) znuC 1519350..1520105 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  PAE60_RS08470 (PAE60_008470) znuA 1520184..1521116 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  PAE60_RS08475 (PAE60_008475) mepM 1521132..1522454 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=749283 PAE60_RS08455 WP_000568519.1 1517411..1518421(+) (ruvB) [Escherichia coli strain C-SRM-3]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=749283 PAE60_RS08455 WP_000568519.1 1517411..1518421(+) (ruvB) [Escherichia coli strain C-SRM-3]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGTGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494