Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   OF800_RS02530 Genome accession   NZ_CP109636
Coordinates   492501..494735 (+) Length   744 a.a.
NCBI ID   WP_264308513.1    Uniprot ID   A0AA46TWH1
Organism   Lactococcus garvieae strain Alexii-6_2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487501..499735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OF800_RS02515 (OF800_02515) - 488876..490432 (+) 1557 WP_004259192.1 ABC-F family ATP-binding cassette domain-containing protein -
  OF800_RS02520 (OF800_02520) - 490598..491773 (+) 1176 WP_264308512.1 SLC13 family permease -
  OF800_RS02525 (OF800_02525) - 491831..492481 (+) 651 WP_042217284.1 helix-hairpin-helix domain-containing protein -
  OF800_RS02530 (OF800_02530) comEC 492501..494735 (+) 2235 WP_264308513.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  OF800_RS02535 (OF800_02535) - 494866..495642 (+) 777 WP_042217333.1 alpha/beta hydrolase family protein -
  OF800_RS02540 (OF800_02540) - 495838..496053 (+) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  OF800_RS02545 (OF800_02545) atpB 496108..496821 (+) 714 WP_004259176.1 F0F1 ATP synthase subunit A -
  OF800_RS02550 (OF800_02550) atpF 496835..497341 (+) 507 WP_004259173.1 F0F1 ATP synthase subunit B -
  OF800_RS02555 (OF800_02555) - 497356..497883 (+) 528 WP_014024339.1 F0F1 ATP synthase subunit delta -
  OF800_RS02560 (OF800_02560) atpA 497949..499457 (+) 1509 WP_004259170.1 F0F1 ATP synthase subunit alpha -

Sequence

Protein


Download         Length: 744 a.a.        Molecular weight: 86042.98 Da        Isoelectric Point: 9.6662

>NTDB_id=748686 OF800_RS02530 WP_264308513.1 492501..494735(+) (comEC) [Lactococcus garvieae strain Alexii-6_2]
MKQKFSLISFVYLLVFVYFLIFSFSWPLLILSLFSLFIAFYRQYYSVLVLLLCFCLFLLMIKKETEVQERKQPEKISKIT
PYMDTLQVNGSRLSFRGEVNGNDFQAFYTLTSEKEKEYFKKLAVKGTFYLEGTLEIPEEQRNFSGFDYRKYLRTQGVYRL
LKIEKIHGFEEKQDFDLRLVRRKAILWAQEHFPSPMSSYMTGLLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIE
LLRNILLRLGLSQKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPLKNSSDRFSVTFLLLMVFSPYFLLTTGGQLTML
YSFLITFFSRKFKELKGLKKSLLDSTVLAVGVLPLLIFHFHSFQPLSIILTLLFSLIFDFLLLPGLLFVFLLSLFGTALT
LFNPFFIVSESIVRWVGDLFDQPIVFGKPNLIFLLLLFMLSGLALDFYKKRNWNLSIIIVLFLLFFFVKNPKHPSITMVD
VGQGDSILLQDSWNSQNILIDTGGRLKIRADEQWKEGIYKSNAENTLLPYLRARGVSKIDVLIVTHPDEDHIGDLESVAQ
KVPIKNVYVSASALEKKSFAKKLSRLNSQIHIVQEGDKLPIFNSYLWFLTTGVEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEVRLQEEYPHLEVDVLKVGHHGSKTSSKEAFLEAINPKLALISAGKNNRYKHPNQETLQQFAARGIKIYRTDQQGA
IRLLYKKGSWHIQTVKELWVQKLL

Nucleotide


Download         Length: 2235 bp        

>NTDB_id=748686 OF800_RS02530 WP_264308513.1 492501..494735(+) (comEC) [Lactococcus garvieae strain Alexii-6_2]
ATGAAACAAAAATTTTCGCTTATCTCTTTCGTTTATCTCTTAGTCTTTGTTTATTTTCTTATTTTTAGTTTTTCATGGCC
TTTACTTATTCTTAGTCTCTTTAGTCTCTTCATCGCTTTTTATCGTCAATACTATAGTGTGTTAGTTCTTTTACTGTGCT
TTTGTTTATTCTTACTCATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACC
CCTTATATGGATACACTTCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAATGATTTTCAAGCTTT
TTACACCTTGACCTCGGAAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACCTTGAAGGTACTT
TGGAGATTCCAGAAGAGCAAAGAAATTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACTCAAGGGGTCTATCGTCTT
CTTAAAATAGAAAAAATTCATGGCTTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTG
GGCTCAGGAACATTTCCCTTCTCCTATGTCGTCCTATATGACAGGGCTTCTTTTTGGGTGGTTGGATAAAGACTTTGAAG
AAATGGGGGAGCTTTACACCAGCCTTGGTATTATCCATTTATTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAA
CTTCTTCGAAATATTTTACTGCGGCTTGGCTTATCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTA
TGCATTTCTTACAGGCTTATCTCTTTCAATCTTACGCGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATA
GATTTTCTGTCACTTTTCTTCTTTTAATGGTGTTCAGTCCTTATTTCCTGCTGACAACAGGAGGACAACTCACGATGCTT
TATTCTTTCTTAATCACATTTTTTTCACGAAAGTTCAAAGAACTAAAAGGATTAAAAAAATCGTTACTTGATTCTACCGT
CCTAGCTGTTGGTGTTCTTCCGCTTTTAATTTTTCACTTCCATAGTTTTCAGCCATTATCGATTATCTTGACTTTATTAT
TTAGTCTGATTTTTGATTTTTTACTTCTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTACTGCTCTCACG
TTATTTAATCCTTTTTTTATTGTTTCAGAATCAATAGTAAGATGGGTGGGCGATTTATTTGACCAGCCGATTGTCTTTGG
CAAACCCAACCTTATTTTTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGGAACTGGA
ACTTGAGCATAATAATTGTCCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGAT
GTGGGACAAGGAGACAGTATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGCGGGAGATTAAA
AATAAGAGCTGATGAGCAGTGGAAGGAGGGAATCTATAAGAGCAATGCCGAAAATACACTTCTGCCTTATCTGAGAGCGC
GTGGCGTGAGTAAGATAGATGTTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAA
CAGTCAGATTCACATTGTGCAGGAGGGGGACAAGCTCCCCATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGAGTTG
AAGGTAAAAGCGATAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGTGACTTA
GAAGAAGAGGGAGAGGTGCGGCTCCAGGAAGAATATCCCCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAG
TAAAACTTCTTCAAAGGAAGCTTTTTTAGAAGCCATCAACCCTAAGCTCGCTCTTATTTCGGCTGGAAAGAATAATCGAT
ATAAACATCCCAATCAAGAAACGCTCCAACAATTCGCTGCACGAGGCATAAAAATTTATCGTACAGACCAGCAAGGGGCA
ATCCGTCTCCTTTATAAAAAAGGCAGTTGGCACATTCAAACAGTAAAGGAGCTTTGGGTACAAAAACTGTTATAG

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(481-689)

Domain of unknown function (DUF4131) (DUF4131)

(23-166)

Competence protein (Competence)

(203-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

52.797

98.522

0.52
  comEC/celB Streptococcus mitis SK321

45.248

100

0.454
  comEC/celB Streptococcus mitis NCTC 12261

43.817

100

0.438
  comEC/celB Streptococcus pneumoniae Rx1

43.373

100

0.435
  comEC/celB Streptococcus pneumoniae D39

43.373

100

0.435
  comEC/celB Streptococcus pneumoniae R6

43.373

100

0.435
  comEC/celB Streptococcus pneumoniae TIGR4

43.472

99.866

0.434