Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   OF800_RS00860 Genome accession   NZ_CP109636
Coordinates   173182..174201 (+) Length   339 a.a.
NCBI ID   WP_264308684.1    Uniprot ID   A0AA46YUD6
Organism   Lactococcus garvieae strain Alexii-6_2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168182..179201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OF800_RS00840 (OF800_00840) - 168707..170161 (-) 1455 WP_264308318.1 GH25 family lysozyme -
  OF800_RS00845 (OF800_00845) guaB 170330..171811 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  OF800_RS00850 (OF800_00850) rpsU 171934..172110 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  OF800_RS00855 (OF800_00855) comGA 172299..173243 (+) 945 WP_264308683.1 competence type IV pilus ATPase ComGA Machinery gene
  OF800_RS00860 (OF800_00860) comGB 173182..174201 (+) 1020 WP_264308684.1 competence type IV pilus assembly protein ComGB Machinery gene
  OF800_RS00865 (OF800_00865) comYC 174220..174525 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  OF800_RS00870 (OF800_00870) comGD 174500..174916 (+) 417 WP_264308320.1 competence type IV pilus minor pilin ComGD -
  OF800_RS00875 (OF800_00875) comGE 174948..175181 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  OF800_RS00880 (OF800_00880) comGF 175162..175602 (+) 441 WP_338129513.1 competence type IV pilus minor pilin ComGF -
  OF800_RS00885 (OF800_00885) - 175611..175772 (+) 162 WP_264308322.1 hypothetical protein -
  OF800_RS00890 (OF800_00890) - 175835..176680 (+) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  OF800_RS00895 (OF800_00895) - 176766..177635 (-) 870 WP_264308323.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39276.98 Da        Isoelectric Point: 9.9313

>NTDB_id=748670 OF800_RS00860 WP_264308684.1 173182..174201(+) (comGB) [Lactococcus garvieae strain Alexii-6_2]
MDLSQLLQLRTKKLSLAKQIKLIQLINNLFKSGFHLSEIIDFLERSGLTEAYFISKMRAGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGFLFSLLIVFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDRGQKFEDALRLSPIFTKELALIVEYGELKSKLGKELMVFSEESWALFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=748670 OF800_RS00860 WP_264308684.1 173182..174201(+) (comGB) [Lactococcus garvieae strain Alexii-6_2]
ATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATAAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGCAGGTTTACTCAACGGTCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GGTTAAAAAATTATTTACTACCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACTCTC
TTTTTAGGTTTCCTCTTCAGTTTGCTTATTGTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTGATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCTCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGATGCCCTTCGACTTTCCCCCATATTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGTCAAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGGCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

52.976

99.115

0.525
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comYB Streptococcus gordonii str. Challis substr. CH1

52.381

99.115

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae Rx1

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae D39

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

52.239

98.82

0.516
  comYB Streptococcus mutans UA140

51.111

92.92

0.475
  comYB Streptococcus mutans UA159

50.794

92.92

0.472