Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   FJMB_RS10275 Genome accession   NZ_AP024687
Coordinates   2185548..2186558 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain FUJ80154     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2180548..2191558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJMB_RS10245 (FJMB80154_19810) nudB 2182272..2182724 (+) 453 WP_077248299.1 dihydroneopterin triphosphate diphosphatase -
  FJMB_RS10250 (FJMB80154_19820) yebC 2182753..2183493 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  FJMB_RS10255 (FJMB80154_19830) ruvC 2183528..2184049 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  FJMB_RS10260 (FJMB80154_19840) yebB 2184051..2184653 (-) 603 WP_022645808.1 YebB family permuted papain-like enzyme -
  FJMB_RS10265 yobI 2184724..2184789 (+) 66 WP_001386853.1 stress response small protein YobI -
  FJMB_RS10270 (FJMB80154_19850) ruvA 2184928..2185539 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  FJMB_RS10275 (FJMB80154_19860) ruvB 2185548..2186558 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  FJMB_RS10280 (FJMB80154_19870) znuB 2186705..2187490 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  FJMB_RS10285 (FJMB80154_19880) znuC 2187487..2188242 (-) 756 WP_022645806.1 zinc ABC transporter ATP-binding protein ZnuC -
  FJMB_RS10290 (FJMB80154_19890) znuA 2188321..2189253 (+) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -
  FJMB_RS10295 (FJMB80154_19900) mepM 2189269..2190591 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=74771 FJMB_RS10275 WP_000568522.1 2185548..2186558(+) (ruvB) [Escherichia coli strain FUJ80154]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=74771 FJMB_RS10275 WP_000568522.1 2185548..2186558(+) (ruvB) [Escherichia coli strain FUJ80154]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAACCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGGGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTAGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGACTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCACCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment