Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   SAGCMC97051_RS02435 Genome accession   NZ_AP020310
Coordinates   398688..400643 (+) Length   651 a.a.
NCBI ID   WP_000614568.1    Uniprot ID   A0A076Z512
Organism   Streptococcus agalactiae strain GCMC97051     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 393688..405643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAGCMC97051_RS02420 (SAGCMC97051_04110) fmt 395666..396601 (+) 936 WP_000163893.1 methionyl-tRNA formyltransferase -
  SAGCMC97051_RS02425 (SAGCMC97051_04120) rsmB 396591..397913 (+) 1323 WP_237395040.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SAGCMC97051_RS02430 (SAGCMC97051_04130) - 397951..398688 (+) 738 WP_000406233.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SAGCMC97051_RS02435 (SAGCMC97051_04140) pknB 398688..400643 (+) 1956 WP_000614568.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  SAGCMC97051_RS02440 (SAGCMC97051_04150) liaF 400804..401499 (+) 696 WP_000714456.1 cell wall-active antibiotics response protein LiaF -
  SAGCMC97051_RS02445 (SAGCMC97051_04160) - 401496..402515 (+) 1020 WP_000716170.1 sensor histidine kinase -
  SAGCMC97051_RS02450 (SAGCMC97051_04170) - 402508..403149 (+) 642 WP_000357892.1 response regulator transcription factor -
  SAGCMC97051_RS02455 (SAGCMC97051_04180) - 403191..404591 (+) 1401 WP_237395041.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SAGCMC97051_RS02460 (SAGCMC97051_04190) - 404593..404949 (+) 357 WP_000689608.1 S1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 651 a.a.        Molecular weight: 70799.56 Da        Isoelectric Point: 9.6759

>NTDB_id=74532 SAGCMC97051_RS02435 WP_000614568.1 398688..400643(+) (pknB) [Streptococcus agalactiae strain GCMC97051]
MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVLRTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIG
EEDGQQFLVMEYVDGFDLKKYIQDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSILAENKSVP
QALENIVIKATAKKLTDRYKTTYEMGRDLSTALSSTRHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTE
KITPDSASNDKTKSKKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENSGLKVGAIR
NIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNYKDILKDLQGKGVKKSLIKVKRKINNDYTTG
TILAQSLPEGTSFNPDGNKKLTLTVAVNDPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQTVVTPPSSSKI
ASQDPYYGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDSSSTSTSSSTLPSDSTT
NTGTANNPLTQ

Nucleotide


Download         Length: 1956 bp        

>NTDB_id=74532 SAGCMC97051_RS02435 WP_000614568.1 398688..400643(+) (pknB) [Streptococcus agalactiae strain GCMC97051]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGGATCCTTAAATCAATCGGACGAGGTGGAATGGCGGATGTTTA
TCTAGCTCGAGATCTAATTTTAGATAATGAAGAGGTCGCAATAAAAGTATTGCGAACCAATTATCAAACAGATCAGATTG
CGGTTGCTCGCTTCCAGAGAGAAGCACGAGCAATGGCTGAGCTGACACATCCTAATATTGTCGCTATTCGTGATATAGGT
GAAGAAGACGGACAGCAGTTCTTAGTAATGGAATATGTTGATGGATTCGATTTAAAAAAGTATATTCAAGACAATGCACC
CCTATCAAATAATGAAGTTGTTCGAATCATGAATGAAGTCCTTTCAGCCATGTCACTGGCACATCAGAAAGGTATCGTAC
ACCGTGATTTAAAACCTCAAAATATTTTATTAACTAAAAAAGGGACTGTAAAAGTTACAGATTTCGGTATTGCGGTAGCG
TTTGCTGAAACTAGTTTAACGCAAACGAATTCTATGTTAGGTAGTGTTCATTATTTATCGCCAGAGCAGGCGCGTGGTTC
AAAAGCGACTGTTCAGAGTGATATTTATGCCATGGGAATCATGCTTTTTGAAATGTTAACTGGCCATATTCCTTATGATG
GGGATAGTGCAGTTACTATTGCCCTACAGCACTTCCAAAAACCACTACCATCAATCCTTGCAGAAAATAAAAGCGTTCCA
CAAGCTTTGGAAAACATTGTCATTAAAGCAACAGCTAAAAAGCTAACAGATCGCTATAAGACAACTTATGAGATGGGACG
TGATTTATCAACTGCTCTAAGCTCGACACGTCACCGTGAACCAAAACTTGTTTTTAATGATACTGAGAGTACTAAGACAC
TACCCAAAGTAACATCTACAGTATCATCTCTAACGACAGAACAACTGTTGCGTAATCAAAAACAGGCCAAAACAACAGAA
AAAATAACTCCTGATTCTGCGAGTAACGATAAAACGAAGTCTAAAAAGAAGGCTTCTCATCGACTTCTTGGTACAATCAT
GAAATTATTCTTTGCACTCTGTGTTGTAGGAATAATTGTATTTGCTTATAAAATTTTAGTAAGTCCTACGACTATTCGTG
TTCCAGATGTTAGCAACAAAACAGTAGCTCAAGCAAAAATGACATTGGAAAATTCTGGATTGAAAGTAGGAGCTATTCGT
AATATTGAGAGTGATAGTGTTTCAGAAGGACTTGTCGTAAAAACAGACCCTGCTGCAGGACGTTCTCGCAGAGAAGGGGC
TAAAGTTAATCTTTATATTGCCACTCCAAACAAGAGTTTCACTCTAGGTAATTATAAGGAACATAATTACAAAGATATCC
TCAAAGATCTTCAAGGCAAAGGTGTTAAAAAATCTTTAATCAAGGTTAAGCGTAAAATTAATAATGATTATACTACTGGT
ACAATCTTAGCACAGTCGCTTCCAGAAGGAACATCTTTTAATCCTGATGGTAATAAGAAATTAACACTTACGGTTGCTGT
TAATGATCCAATGATTATGCCTGATGTAACAGGTATGACTGTTGGAGAAGTAATAGAAACCTTGACAGACCTCGGATTAG
ATGCAGATAATCTTGTCTTCTATCAAATGCAAAATGGCGTTTATCAAACAGTAGTAACACCTCCATCTTCAAGTAAGATA
GCAAGTCAGGATCCTTATTACGGAGGAGAAGTTGGATTAAGGAGAGGAGATAAAGTTAAGCTGTATCTTCTTGGTTCTAA
AACAACGAATAACTCATCATCTACGCCAATAGATTCTTCGGCTTCAAGCTCAACAGGAACAACTACCTCGGATTCTGTAA
GCTCATCAACGGATGCTAGCACTTCTGATAGTAGTAGCACTTCTACAAGTAGTTCAACACTACCAAGTGATAGTACAACT
AATACAGGAACAGCTAACAATCCATTAACTCAATAA

Domains

Predicted by InterproScan.

PASTA domain (PASTA)

(511-583)

PASTA domain (PASTA)

(373-434)

PASTA domain (PASTA)

(441-506)

Protein kinase domain (Pkinase)

(12-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A076Z512

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

60.959

89.708

0.547
  stkP/pknB Streptococcus salivarius strain HSISS4

60.9

88.786

0.541
  stkP Streptococcus pneumoniae TIGR4

59.612

79.109

0.472
  stkP Streptococcus pneumoniae D39

59.417

79.109

0.47
  stkP Streptococcus pneumoniae R6

59.417

79.109

0.47
  stkP Streptococcus pneumoniae Rx1

59.223

79.109

0.469

Multiple sequence alignment