Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QEN53_RS10080 Genome accession   NZ_CP123024
Coordinates   2079647..2080657 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 20-16     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2074647..2085657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEN53_RS10050 nudB 2076371..2076823 (+) 453 WP_001443602.1 dihydroneopterin triphosphate diphosphatase -
  QEN53_RS10055 yebC 2076852..2077592 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QEN53_RS10060 ruvC 2077627..2078148 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  QEN53_RS10065 yebB 2078150..2078752 (-) 603 WP_001024930.1 YebB family permuted papain-like enzyme -
  QEN53_RS10070 yobI 2078823..2078888 (+) 66 WP_001386853.1 stress response small protein YobI -
  QEN53_RS10075 ruvA 2079027..2079638 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  QEN53_RS10080 ruvB 2079647..2080657 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QEN53_RS10085 znuB 2080804..2081589 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  QEN53_RS10090 znuC 2081586..2082341 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  QEN53_RS10095 znuA 2082420..2083352 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  QEN53_RS10100 mepM 2083368..2084690 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=745068 QEN53_RS10080 WP_000568519.1 2079647..2080657(+) (ruvB) [Escherichia coli strain 20-16]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=745068 QEN53_RS10080 WP_000568519.1 2079647..2080657(+) (ruvB) [Escherichia coli strain 20-16]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494