Detailed information NTG0744986

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: QEN55_RS10115
• Genome accession: NZ_CP123017
• Coordinates: 2086926..2087936 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli strain 19-7
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2081926..2092936

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEN55_RS10085	nudB	2083650..2084102 (+)	453	WP_001443602.1	dihydroneopterin triphosphate diphosphatase	-
QEN55_RS10090	yebC	2084131..2084871 (+)	741	WP_000907248.1	YebC/PmpR family DNA-binding transcriptional regulator	-
QEN55_RS10095	ruvC	2084906..2085427 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
QEN55_RS10100	yebB	2085429..2086031 (-)	603	WP_001024930.1	YebB family permuted papain-like enzyme	-
QEN55_RS10105	yobI	2086102..2086167 (+)	66	WP_001386853.1	stress response small protein YobI	-
QEN55_RS10110	ruvA	2086306..2086917 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
QEN55_RS10115	ruvB	2086926..2087936 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
QEN55_RS10120	znuB	2088083..2088868 (-)	786	WP_000571479.1	zinc ABC transporter permease subunit ZnuB	-
QEN55_RS10125	znuC	2088865..2089620 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
QEN55_RS10130	znuA	2089699..2090631 (+)	933	WP_001342995.1	zinc ABC transporter substrate-binding protein ZnuA	-
QEN55_RS10135	mepM	2090647..2091969 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=744986 QEN55_RS10115 WP_000568519.1 2086926..2087936(+) (ruvB) [Escherichia coli strain 19-7]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=744986 QEN55_RS10115 WP_000568519.1 2086926..2087936(+) (ruvB) [Escherichia coli strain 19-7]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494