Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K5706_RS02880 Genome accession   NZ_AP019849
Coordinates   589163..589636 (-) Length   157 a.a.
NCBI ID   WP_275946047.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya249     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 584163..594636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5706_RS02860 (VA249_04810) coaE 584672..585286 (-) 615 WP_221068091.1 dephospho-CoA kinase -
  K5706_RS02865 (VA249_04820) pilD 585287..586156 (-) 870 WP_221070944.1 prepilin peptidase Machinery gene
  K5706_RS02870 (VA249_04830) pilC 586221..587444 (-) 1224 WP_221070946.1 type II secretion system F family protein Machinery gene
  K5706_RS02875 (VA249_04840) pilB 587478..589163 (-) 1686 WP_221070948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5706_RS02880 (VA249_04850) pilA 589163..589636 (-) 474 WP_275946047.1 pilin Machinery gene
  K5706_RS02885 (VA249_04860) nadC 589859..590746 (-) 888 WP_221070950.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5706_RS02890 (VA249_04870) ampD 590839..591396 (+) 558 WP_221070952.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5706_RS02895 (VA249_04880) pdhR 591792..592559 (+) 768 WP_221070954.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16805.13 Da        Isoelectric Point: 4.5482

>NTDB_id=74394 K5706_RS02880 WP_275946047.1 589163..589636(-) (pilA) [Vibrio alfacsensis strain 04Ya249]
MRTNKHMKQKGFTLIELMIVVAIIGILAAFAVPAYQDYTRKATMAEFPKVASAIKLAVELCASESEQSNFASNCISSVSG
AMTSVPKEVTLNNIKIYAQAGDLTSGAVEVIAEATADKGPIATSEKYVMAATFNDNGIEWSSSCFDANDDQQTTYCP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=74394 K5706_RS02880 WP_275946047.1 589163..589636(-) (pilA) [Vibrio alfacsensis strain 04Ya249]
ATGAGAACGAATAAACACATGAAACAAAAAGGCTTTACGCTCATTGAGTTAATGATCGTAGTTGCAATTATTGGCATTTT
GGCGGCATTTGCAGTCCCTGCTTACCAAGACTACACAAGAAAAGCAACGATGGCAGAATTTCCTAAAGTTGCTTCAGCCA
TAAAGCTTGCCGTAGAACTTTGTGCTAGTGAAAGTGAGCAAAGCAACTTTGCTTCCAATTGCATAAGCTCAGTGAGTGGA
GCAATGACATCGGTCCCAAAGGAAGTAACACTAAATAATATAAAAATTTATGCACAAGCAGGAGATTTAACCTCTGGTGC
AGTAGAGGTGATAGCTGAAGCAACCGCAGATAAAGGGCCAATTGCGACGAGCGAAAAGTACGTAATGGCAGCAACATTTA
ATGATAATGGCATTGAATGGAGCAGTTCCTGTTTTGATGCCAACGATGACCAGCAAACAACTTACTGCCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

54.717

100

0.554
  pilA2 Legionella pneumophila str. Paris

42.647

86.624

0.369
  pilA2 Legionella pneumophila strain ERS1305867

41.912

86.624

0.363

Multiple sequence alignment