Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GL2_RS05505 Genome accession   NZ_AP019807
Coordinates   1280783..1281859 (-) Length   358 a.a.
NCBI ID   WP_143729656.1    Uniprot ID   A0A510IQ71
Organism   Microbulbifer sp. GL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1275783..1286859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL2_RS05480 (GL2_11130) - 1276345..1276836 (-) 492 WP_232053773.1 shikimate kinase -
  GL2_RS05485 (GL2_11140) pilQ 1276866..1279034 (-) 2169 WP_143729652.1 type IV pilus secretin PilQ -
  GL2_RS05490 (GL2_11150) - 1279034..1279564 (-) 531 WP_143729653.1 pilus assembly protein PilP -
  GL2_RS05495 (GL2_11160) - 1279561..1280181 (-) 621 WP_143729654.1 type 4a pilus biogenesis protein PilO -
  GL2_RS05500 (GL2_11170) - 1280194..1280781 (-) 588 WP_143729655.1 PilN domain-containing protein -
  GL2_RS05505 (GL2_11180) pilM 1280783..1281859 (-) 1077 WP_143729656.1 pilus assembly protein PilM Machinery gene
  GL2_RS05510 (GL2_11190) - 1282007..1284667 (+) 2661 WP_143729657.1 penicillin-binding protein 1A -
  GL2_RS05515 (GL2_11200) - 1284791..1285273 (-) 483 WP_143729658.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39050.22 Da        Isoelectric Point: 4.3231

>NTDB_id=74211 GL2_RS05505 WP_143729656.1 1280783..1281859(-) (pilM) [Microbulbifer sp. GL-2]
MGIFPFLDKGPKAMLGLDISSTAVKLLELSRNGNKYRVESYAVEPLPPNAVVDKNINDVGATAEAIRKVVRRSKTRLRRA
AVAVSGSAVITKTLEMPADLSDEDLEARIALEADQYIPYPLEEVSLDFEVQGASEKGEEQVEVLLAACRSENIEQRISAL
SEADLEAGVVDVEAYAIERTYTLLEDQFPPQEQMVVAVVDIGATMSALSVLIDGKTVYTREQLFGGRQLTDEIQRRYGLS
AEEASLAKHQGGSGLPDDYYAEVLEPFRDAVIQQVTRSLQFFYSSTSYSDVDYILLSGGVAAMEGLSELVGEKLGKPTLV
ADPFHGMSLSSRINRQSLANEAPALMIAAGLAMREKEY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=74211 GL2_RS05505 WP_143729656.1 1280783..1281859(-) (pilM) [Microbulbifer sp. GL-2]
ATGGGAATTTTCCCTTTTTTGGACAAAGGCCCTAAGGCCATGCTGGGACTCGATATTAGCTCGACCGCAGTGAAGTTGCT
GGAGCTAAGTCGCAATGGCAATAAATATCGTGTCGAAAGTTATGCCGTGGAACCCCTGCCACCCAATGCGGTGGTGGACA
AGAACATCAATGATGTCGGTGCCACAGCTGAGGCTATTCGTAAGGTGGTACGCCGATCTAAAACCCGTTTGCGCCGCGCT
GCGGTAGCCGTTTCTGGCTCGGCTGTGATCACCAAGACCCTGGAAATGCCTGCGGATCTTTCCGATGAGGATCTGGAGGC
GCGTATAGCTCTTGAGGCAGACCAGTACATTCCCTACCCGCTTGAGGAGGTGAGCCTGGACTTCGAAGTGCAGGGGGCAT
CTGAAAAGGGCGAGGAGCAGGTGGAAGTTCTGCTCGCGGCATGTCGCAGCGAGAATATTGAGCAGCGGATTTCCGCTCTC
AGCGAGGCCGATCTGGAGGCTGGTGTAGTCGATGTGGAAGCTTATGCCATAGAGCGGACATACACCCTGCTGGAGGATCA
ATTCCCACCTCAGGAGCAAATGGTTGTTGCAGTAGTTGATATTGGCGCCACCATGAGCGCCTTGTCAGTTCTTATTGATG
GCAAAACTGTTTACACACGAGAACAGCTTTTTGGTGGTCGCCAGCTTACCGACGAAATCCAGCGCCGCTATGGTCTTTCC
GCGGAGGAAGCCTCTCTGGCCAAGCACCAGGGCGGTAGTGGATTACCGGATGATTACTATGCAGAGGTACTGGAGCCCTT
TCGGGATGCGGTAATCCAGCAGGTTACCCGCTCATTACAGTTCTTTTACTCTTCCACTTCCTACAGCGATGTGGATTACA
TCCTACTCAGCGGTGGCGTTGCAGCAATGGAGGGGTTATCCGAGTTAGTTGGTGAGAAATTGGGTAAACCCACTCTAGTC
GCTGATCCTTTCCATGGGATGAGCCTCTCCTCGCGAATTAATCGCCAATCTCTGGCCAATGAGGCACCTGCGTTGATGAT
TGCCGCAGGGTTAGCGATGCGGGAGAAGGAGTACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510IQ71

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.867

96.648

0.492
  comM Acinetobacter nosocomialis M2

50.867

96.648

0.492
  comM Acinetobacter baylyi ADP1

47.522

95.81

0.455
  pilM Legionella pneumophila strain ERS1305867

42.697

99.441

0.425

Multiple sequence alignment