Detailed information NTG0741506

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OIO47_RS14310
• Genome accession: NZ_CP107614
• Coordinates: 2960805..2961923 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 1326927-2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2955805..2966923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OIO47_RS14300 (OIO47_14300)	-	2958921..2959613 (-)	693	WP_031959811.1	YggS family pyridoxal phosphate-dependent enzyme	-
OIO47_RS14305 (OIO47_14305)	pilT	2959740..2960777 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OIO47_RS14310 (OIO47_14310)	pilU	2960805..2961923 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OIO47_RS14315 (OIO47_14315)	fur	2961993..2962430 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OIO47_RS14320 (OIO47_14320)	-	2962542..2962940 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OIO47_RS14325 (OIO47_14325)	-	2962995..2963318 (-)	324	WP_000442585.1	RnfH family protein	-
OIO47_RS14330 (OIO47_14330)	-	2963318..2964385 (-)	1068	WP_031959810.1	membrane protein	-
OIO47_RS14335 (OIO47_14335)	-	2964538..2964993 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OIO47_RS14340 (OIO47_14340)	-	2965246..2966094 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=741506 OIO47_RS14310 WP_000347036.1 2960805..2961923(+) (pilU) [Acinetobacter baumannii strain 1326927-2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=741506 OIO47_RS14310 WP_000347036.1 2960805..2961923(+) (pilU) [Acinetobacter baumannii strain 1326927-2]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392