Detailed information NTG0741304

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OIO71_RS14635
• Genome accession: NZ_CP107603
• Coordinates: 3035261..3036379 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 1326595
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3030261..3041379

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OIO71_RS14625 (OIO71_14630)	-	3033377..3034069 (-)	693	WP_031959811.1	YggS family pyridoxal phosphate-dependent enzyme	-
OIO71_RS14630 (OIO71_14635)	pilT	3034196..3035233 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OIO71_RS14635 (OIO71_14640)	pilU	3035261..3036379 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OIO71_RS14640 (OIO71_14645)	fur	3036449..3036886 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OIO71_RS14645 (OIO71_14650)	-	3036998..3037396 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OIO71_RS14650 (OIO71_14655)	-	3037451..3037774 (-)	324	WP_000442585.1	RnfH family protein	-
OIO71_RS14655 (OIO71_14660)	-	3037774..3038841 (-)	1068	WP_031959810.1	membrane protein	-
OIO71_RS14660 (OIO71_14665)	-	3038994..3039449 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OIO71_RS14665 (OIO71_14670)	-	3039702..3040550 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=741304 OIO71_RS14635 WP_000347036.1 3035261..3036379(+) (pilU) [Acinetobacter baumannii strain 1326595]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=741304 OIO71_RS14635 WP_000347036.1 3035261..3036379(+) (pilU) [Acinetobacter baumannii strain 1326595]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392