Detailed information NTG0741141

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OIO65_RS14550
• Genome accession: NZ_CP107595
• Coordinates: 3015563..3016681 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 1326581-1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3010563..3021681

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OIO65_RS14540 (OIO65_14545)	-	3013679..3014371 (-)	693	WP_031959811.1	YggS family pyridoxal phosphate-dependent enzyme	-
OIO65_RS14545 (OIO65_14550)	pilT	3014498..3015535 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OIO65_RS14550 (OIO65_14555)	pilU	3015563..3016681 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OIO65_RS14555 (OIO65_14560)	fur	3016751..3017188 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OIO65_RS14560 (OIO65_14565)	-	3017300..3017698 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OIO65_RS14565 (OIO65_14570)	-	3017753..3018076 (-)	324	WP_000442585.1	RnfH family protein	-
OIO65_RS14570 (OIO65_14575)	-	3018076..3019143 (-)	1068	WP_031959810.1	membrane protein	-
OIO65_RS14575 (OIO65_14580)	-	3019296..3019751 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OIO65_RS14580 (OIO65_14585)	-	3020004..3020852 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=741141 OIO65_RS14550 WP_000347036.1 3015563..3016681(+) (pilU) [Acinetobacter baumannii strain 1326581-1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=741141 OIO65_RS14550 WP_000347036.1 3015563..3016681(+) (pilU) [Acinetobacter baumannii strain 1326581-1]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392