Detailed information NTG0740653

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: QC805_RS09205
• Genome accession: NZ_CP122496
• Coordinates: 1891326..1892336 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli strain a7
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1886326..1897336

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QC805_RS09175	nudB	1888050..1888502 (+)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-
QC805_RS09180	yebC	1888531..1889271 (+)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
QC805_RS09185	ruvC	1889306..1889827 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
QC805_RS09190	yebB	1889829..1890431 (-)	603	WP_001024904.1	YebB family permuted papain-like enzyme	-
QC805_RS09195	yobI	1890502..1890567 (+)	66	WP_072094247.1	stress response small protein YobI	-
QC805_RS09200	ruvA	1890706..1891317 (+)	612	WP_000580327.1	Holliday junction branch migration protein RuvA	-
QC805_RS09205	ruvB	1891326..1892336 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
QC805_RS09210	znuB	1892483..1893268 (-)	786	WP_000571479.1	zinc ABC transporter permease subunit ZnuB	-
QC805_RS09215	znuC	1893265..1894020 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
QC805_RS09220	znuA	1894099..1895031 (+)	933	WP_284614150.1	zinc ABC transporter substrate-binding protein ZnuA	-
QC805_RS09225	mepM	1895047..1896369 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=740653 QC805_RS09205 WP_000568519.1 1891326..1892336(+) (ruvB) [Escherichia coli strain a7]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=740653 QC805_RS09205 WP_000568519.1 1891326..1892336(+) (ruvB) [Escherichia coli strain a7]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494